WEKO3
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An Ex Vivo Model Employing Keloid-Derived Cell–Seeded Collagen Sponges for Therapy Development
http://hdl.handle.net/10069/31452
http://hdl.handle.net/10069/31452e4948755-bd0c-4f15-ab52-63d55df85879
名前 / ファイル | ライセンス | アクション |
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JID133_386.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-09-01 | |||||
タイトル | ||||||
タイトル | An Ex Vivo Model Employing Keloid-Derived Cell–Seeded Collagen Sponges for Therapy Development | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chondroitin ABC lyase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | collagen sponge | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glycosaminoglycan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | interleukin 1beta | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proteoglycan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | versican | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | animal experiment | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | article | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fibroblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in vitro study | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | keloid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mesenchyme cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mouse | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nonhuman | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pathogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | priority journal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | real time polymerase chain reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | upregulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adult | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Animals | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chondroitin ABC Lyase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Disease Models, Animal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Extracellular Matrix | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Female | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibroblasts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene Expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glycosaminoglycans | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Humans | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Interleukin-1beta | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Keloid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Male | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mice, Nude | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Primary Cell Culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Surgical Sponges | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Up-Regulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Versicans | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yagi, Yosuke
× Yagi, Yosuke× Muroga, Eri× Naitoh, Motoko× Isogai, Zenzo× Matsui, Seiya× Ikehara, Susumu× Suzuki, Shigehiko× Miyachi, Yoshiki× Utani, Atsushi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The most distinctive feature of keloid is the extreme deposition of extracellular matrix, including collagens and proteoglycans (PGs). The focus of this study was the PG versican, which presumably defines keloid volume because of its ability to retain large amounts of water through its component glycosaminoglycans (GAGs). The excessive deposition of versican in keloids was examined by immunohistochemical analysis and by upregulation of the versican gene in these lesions by real-time PCR. The latter showed that mesenchymal cells derived from keloid lesion (KL) cells continue to exhibit above-normal versican production in culture. To establish a model of GAG deposition in keloids, collagen sponges seeded with KL cells (KL-SPos) were implanted in the subcutaneous space of nude mice. After 1 month, the KL-SPos were significantly heavier than the fibroblast (Fb)-seeded sponges (Fb-SPos). This ex vivo model was subsequently used to examine an inhibitory ability of IL-1β that was identified to reduce versican in vitro. IL-1β or chondroitinase ABC, when injected directly, successfully reduced the weight of the KL-SPos. Thus, on the basis of the change in weight of the seeded sponges, this ex vivo model can be used to test therapies aimed at reducing or inhibiting keloid formation and to study the pathogenesis of this aberrant response. | |||||
書誌情報 |
Journal of Investigative Dermatology 巻 133, 号 2, p. 386-393, 発行日 2013-02 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0022202X | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15231747 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/jid.2012.314 | |||||
権利 | ||||||
権利情報 | © 2013 The Society for Investigative Dermatology. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Investigative Dermatology, 133(2), pp.386-393; 2013 |