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A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies
http://hdl.handle.net/10069/40071
http://hdl.handle.net/10069/4007134b66e34-86b5-4250-a536-30fac32ffb04
名前 / ファイル | ライセンス | アクション |
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VACCINES8_297_.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-29 | |||||
タイトル | ||||||
タイトル | A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dengue | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ELISA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Flavivirus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microneutralization test | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Neutralizing antibodies | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Zika | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Balingit, Jean Claude
× Balingit, Jean Claude× Phu, Ly Minh Huong× Matsuda, Mami× Suzuki, Ryosuke× Hasebe, Futoshi× Morita, Kouichi× Moi, Meng Ling |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Mosquito-borne flavivirus infections, including dengue virus and Zika virus, are major public health threats globally. While the plaque reduction neutralization test (PRNT) is considered the gold standard for determining neutralizing antibody levels to flaviviruses, the assay is time-consuming and laborious. This study, therefore, aimed to develop an enzyme-linked immunosorbent assay (ELISA)-based microneutralization test (EMNT) for the detection of neutralizing antibodies to mosquito-borne flaviviruses. The inhibition of viral growth due to neutralizing antibodies was determined colorimetrically by using EMNT. Given the significance of Fcγ-receptors (FcγR) in antibody-mediated neutralization and antibody-dependent enhancement (ADE) of flavivirus infection, non-FcγR and FcγR-expressing cell lines were used in the EMNT to allow the detection of the sum of neutralizing and immune-enhancing antibody activity as the neutralizing titer. Using anti-flavivirus monoclonal antibodies and clinical samples, the utility of EMNT was evaluated by comparing the end-point titers of the EMNT and the PRNT. The correlation between EMNT and PRNT titers was strong, indicating that EMNT was robust and reproducible. The new EMNT assay combines the biological functional assessment of virus neutralization activity and the technical advantages of ELISA and, is simple, reliable, practical, and could be automated for high-throughput implementation in flavivirus surveillance studies and vaccine trials. | |||||
書誌情報 |
Vaccines 巻 8, 号 2, p. 297, 発行日 2020-06-10 |
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出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2076393X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/vaccines8020297 | |||||
権利 | ||||||
権利情報 | c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Vaccines, 8(2), art.no.297, pp.1-15; 2020 |