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Intracellular Internalization Mechanism of Protein Transfection Reagents
http://hdl.handle.net/10069/29763
http://hdl.handle.net/10069/297636ec78f1e-71c0-4f7f-83b3-1a22f9376aaf
名前 / ファイル | ライセンス | アクション |
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BPBul35_1064.pdf (4.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-11-16 | |||||
タイトル | ||||||
タイトル | Intracellular Internalization Mechanism of Protein Transfection Reagents | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Endocytosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Intracellular internalization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein delivery system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein transfection reagent | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oba, Makoto
× Oba, Makoto× Tanaka, Masakazu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A protein transfection reagent is a powerful tool for elucidating a protein function in a cell, and plays an important role in the fields of cell biology and drug discovery. Many researchers have developed protein delivery systems and several systems are commercially available. In this study, we focus on the biological functions of three commercially available protein transfection reagents, Pro-DeliverIN, Xfect, and TurboFect, especially in their internalization routes by HeLa cells. A cellular uptake study using specific endocytosis inhibitors and confocal laser scanning microscope observation revealed that each reagent was internalized into HeLa cells by different mechanism. It is our hope that the results presented here will help in the choice and use of protein transfection reagents for experiments. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 35, 号 7, p. 1064-1068, 発行日 2012-07-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b12-00001 | |||||
権利 | ||||||
権利情報 | © 2012 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 35(7), pp.1064-1068; 2012 |