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The results are as follows: 1) It is desirable that the sample for ATP determination which is put in proper quantity in a glass vial of the ATP photometer (Labo science, TD-4000) is determined by injecting luciferase reagent from the silinge of the photometer and that the light emitted in ATP determination is assayed by peak light emission. 2) It is necessary that commercial pyruvate kinase and myokinase (adenylate kinase)preparations for conversions of ADP and AMP to ATP are dialyzed for 5 h and that the myokinase after dialysis is immediately used because of the lability. 3) The conversions of ADP and AMP to ATP needed incubation at 30℃ for 30 min. 4) It was able to determine AMP in substituting ADP for ATP which needs for AMP determination. 5) The light emitted in ATP determination decreased with increase of concentration of sea water in samples but the strength of the light emission was proportional to the concentration of ATP at the same concentration of sea water. 6) The conversions of ADP and AMP to ATP were not prevented in sea water samples of adenosine phosphates. 7) Some commercial beef extract contained considerable amount of AMP. 8) Adenosine phosphate concentrations were not able to determine correctly by using cells in bacterial culture. 9) In adenosine phosphate determinations by using the whole of bacterial culture, it is necessary that the analysis is immediately carried out after incubation and that the sampling from the culture define the point of it. 10) It was able to exactly and rapidly determine adenosine phosphates by calibration with standard solution of adenosine phosphates.", "subitem_description_type": "Abstract"}, {"subitem_description": "Kar1\u0026Holm-Hansen^1)によるアデノシンリン酸の分析法と細菌培養液における同物質量の測定法を検討し,次の結果を得た。1)ATPの分析に際しては,ATP試料は測定機器のキ\nューベット側に適量を入れ,蛍光強度はピークの高さから求めることが望ましい。2)ADPおよびAMP量の測定に使用する市販のピルベイトキナーゼとミオキナーゼは約5時間の透析が必要であり,また透析後のミオキナーゼは活性の低下が速やかで,透析後は直ちに使用する必要がある。3)ADPおよびAMPをATPに転換するには30℃ で30分反応する必要がある。4)AMPの分析に必要とされるATPは,ADPを用いても測定が可能であった。5)培地の海水濃度の増加に伴い,ATP測定に伴う蛍光の強度は低下したが,同濃度の海水ではアデノシンリン酸量と測定値は正の相関を示した。6)ADPおよびAMPからATPへの転換は海水試料でも影響はないと考えられた。7)培地成分の牛肉エキスには,特にAMPが多く含まれ\nるものがあった。8)培養液のアデノシンリン酸量を測定するについては,菌体を用いる測定では正しい値を得ることができなかった。9)培養液全体を用いる測定でも,培養液の部分リ培養後に培養温度と異なる温度環境に置くことで測定値が著しく異なった。10)以上の点を考慮し,また測定に際しては各アデノシンリン酸の標準物質を用いることで,迅速に正確に分析することができる", "subitem_description_type": "Abstract"}]}, "item_3_description_64": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "長崎大学水産学部研究報告, v.76, pp.7-14; 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細菌培養液におけるアデノシンリン酸の分析法の検討
http://hdl.handle.net/10069/29790
http://hdl.handle.net/10069/297903a84ab3e-fa05-4ef6-a5cd-6a4b45156a66
名前 / ファイル | ライセンス | アクション |
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suisan76_007.pdf (807.8 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-11-20 | |||||
タイトル | ||||||
タイトル | 細菌培養液におけるアデノシンリン酸の分析法の検討 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | アデノシンリン酸の測定 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adenosine phosphate determinations | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 蛍光 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | luminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 細菌培養液 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacterial culture | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Welly, Mewengkan Hanny
× Welly, Mewengkan Hanny× 石本, 亮× 笠間, 憲太郎× 森井, 秀昭 |
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著者別名 | ||||||
姓名 | Welly, M.H. | |||||
著者別名 | ||||||
姓名 | Ishimoto, Ryo | |||||
著者別名 | ||||||
姓名 | Kasama, Kentaro | |||||
著者別名 | ||||||
姓名 | Morii, Hideaki | |||||
その他のタイトル | ||||||
その他のタイトル | Methodology for adenosine phosphate determinations in bacterial culture. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Method for determination of adenosine phosphates in environmental samples by Karl & Holm-Hansen was reexamined. The results are as follows: 1) It is desirable that the sample for ATP determination which is put in proper quantity in a glass vial of the ATP photometer (Labo science, TD-4000) is determined by injecting luciferase reagent from the silinge of the photometer and that the light emitted in ATP determination is assayed by peak light emission. 2) It is necessary that commercial pyruvate kinase and myokinase (adenylate kinase)preparations for conversions of ADP and AMP to ATP are dialyzed for 5 h and that the myokinase after dialysis is immediately used because of the lability. 3) The conversions of ADP and AMP to ATP needed incubation at 30℃ for 30 min. 4) It was able to determine AMP in substituting ADP for ATP which needs for AMP determination. 5) The light emitted in ATP determination decreased with increase of concentration of sea water in samples but the strength of the light emission was proportional to the concentration of ATP at the same concentration of sea water. 6) The conversions of ADP and AMP to ATP were not prevented in sea water samples of adenosine phosphates. 7) Some commercial beef extract contained considerable amount of AMP. 8) Adenosine phosphate concentrations were not able to determine correctly by using cells in bacterial culture. 9) In adenosine phosphate determinations by using the whole of bacterial culture, it is necessary that the analysis is immediately carried out after incubation and that the sampling from the culture define the point of it. 10) It was able to exactly and rapidly determine adenosine phosphates by calibration with standard solution of adenosine phosphates. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Kar1&Holm-Hansen^1)によるアデノシンリン酸の分析法と細菌培養液における同物質量の測定法を検討し,次の結果を得た。1)ATPの分析に際しては,ATP試料は測定機器のキ ューベット側に適量を入れ,蛍光強度はピークの高さから求めることが望ましい。2)ADPおよびAMP量の測定に使用する市販のピルベイトキナーゼとミオキナーゼは約5時間の透析が必要であり,また透析後のミオキナーゼは活性の低下が速やかで,透析後は直ちに使用する必要がある。3)ADPおよびAMPをATPに転換するには30℃ で30分反応する必要がある。4)AMPの分析に必要とされるATPは,ADPを用いても測定が可能であった。5)培地の海水濃度の増加に伴い,ATP測定に伴う蛍光の強度は低下したが,同濃度の海水ではアデノシンリン酸量と測定値は正の相関を示した。6)ADPおよびAMPからATPへの転換は海水試料でも影響はないと考えられた。7)培地成分の牛肉エキスには,特にAMPが多く含まれ るものがあった。8)培養液のアデノシンリン酸量を測定するについては,菌体を用いる測定では正しい値を得ることができなかった。9)培養液全体を用いる測定でも,培養液の部分リ培養後に培養温度と異なる温度環境に置くことで測定値が著しく異なった。10)以上の点を考慮し,また測定に際しては各アデノシンリン酸の標準物質を用いることで,迅速に正確に分析することができる |
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書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries, Nagasaki University 巻 76, p. 7-14, 発行日 1995-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40002770940 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, v.76, pp.7-14; 1995 |