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コイ腹腔滲出細胞からのマクロファージの調製
http://hdl.handle.net/10069/29831
http://hdl.handle.net/10069/29831d5698ab8-d431-4169-984c-44c49a9ea48b
名前 / ファイル | ライセンス | アクション |
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suisan74-75_051.pdf (5.6 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-11-26 | |||||
タイトル | ||||||
タイトル | コイ腹腔滲出細胞からのマクロファージの調製 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | マクロファージ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | macrophage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 腹腔滲出細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | peritoneal exudate cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 好中球 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | neutrophil | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | コイ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | carp | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 非特異性エステラーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | non-specific esterase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
原, 研治
× 原, 研治× 槌本, 六秀× 橘, 勝康× 長富, 潔× 古場, 久代× 石原, 忠 |
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著者別名 | ||||||
姓名 | Hara, Kenji | |||||
著者別名 | ||||||
姓名 | Tsuchimoto, Mutsuhide | |||||
著者別名 | ||||||
姓名 | Tachibana, Katsuyasu | |||||
著者別名 | ||||||
姓名 | Osatomi, Kiyoshi | |||||
著者別名 | ||||||
姓名 | Koba, Hisayo | |||||
著者別名 | ||||||
姓名 | Ishihara, Tadashi | |||||
その他のタイトル | ||||||
その他のタイトル | Preparation of Macrophage Monolayer from Carp Peritoneal Exudate Cells | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We investigated the preparation of macrophage monolayer from carp peritoneal exudate cells (PEC) to clarify the biosynthesis and processing of cathepsins. PEC collected at 24h interval after elicitation with 6% sodium caseinate or thioglycolate medium were transferred to culture dish in RPMI 1640 (RPMI), and the cells were incubated in CO₂ incubator for 2h. To distinguish macrophage and neutrophil, the adherent cells washed with RPMI to remove non-adherent cells were analyzed histochemically by the staining of two different esterase activities. The number of PEC was maximally increased at 3 days after injection with 15 ml of sodium caseinate, whereas that of macrophages was at 4 days after injection. Most. (about 90%) of cells cultured for 24h were positive for α-Naphthyl butyrate esterase activity but they were not positive for Naphthol AS-D chloroacetate esterase activity, confirming them to be macrophage. They were able to culture for 48h in RPMI containing 5% heat inactivated calf serum. It was possible to prepare two culture dishes of macrophage monolayer (1.33 x 10⁵/cm²) from one carp (800-900g) by this method. | |||||
書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries, Nagasaki University 巻 74・75, p. 51-57, 発行日 1993-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40002770932 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 崎大学水産学部研究報告, v.74・75, pp.51-57; 1993 |