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A single nucleotide polymorphism in 3′-untranslated region contributes to the regulation of toll-like receptor 4 translation
http://hdl.handle.net/10069/29870
http://hdl.handle.net/10069/2987054d8d8b0-46bf-4282-b844-4f456ffaca56
名前 / ファイル | ライセンス | アクション |
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JBC287_25163.pdf (392.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-11-28 | |||||
タイトル | ||||||
タイトル | A single nucleotide polymorphism in 3′-untranslated region contributes to the regulation of toll-like receptor 4 translation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Genetic variation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipopolysaccharides | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luciferase activities | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MicroRNAs | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MRNA level | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Peripheral blood mononuclear cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein level | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Single nucleotide polymorphisms | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Toll-like receptor 4 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transient transfection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Translational regulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Untranslated regions | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sato, Kayo
× Sato, Kayo× Yoshimura, Atsutoshi× Kaneko, Takashi× Ukai, Takashi× Ozaki, Yukio× Nakamura, Hirotaka× Li, Xinyue× Matsumura, Hiroyoshi× Hara, Yoshitaka× Ogata, Yorimasa |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have previously shown that a single nucleotide polymorphism rs11536889 in the 3′-untranslated region (UTR) of TLR4 was associated with periodontitis. In this study the effects of this single nucleotide polymorphism on Toll-like receptor (TLR) 4 expression were investigated. Monocytes from subjects with the C/C genotype expressed higher levels of TLR4 on their surfaces than those from subjects with the other genotypes. Peripheral blood mononuclear cells (PBMCs) from the C/C and G/C subjects secreted higher levels of IL-8 in response to lipopolysaccharide (LPS), a TLR4 ligand, than the cells from the G/G subjects. However, there was no significant difference in TLR4 mRNA levels in PBMCs from the subjects with each genotype. After stimulation with tripalmitoylated CSK 4 (Pam 3CSK 4), TLR4 mRNA levels increased in PBMCs from both the C/C and G/G subjects, whereas TLR4 protein levels increased in PBMCs from the C/C but not G/G subjects. Transient transfection of a series of chimeric luciferase constructs revealed that a fragment of 3′-UTR containing rs11536889 G allele, but not C allele, suppressed luciferase activity induced by LPS or IL-6. Two micro- RNAs, hsa-miR-1236 and hsa-miR-642a, were predicted to bind to rs11536889 G allele. Inhibition of these microRNAs reversed the suppressed luciferase activity. These microRNA inhibitors also up-regulated endogenous TLR4 protein on THP-1 cells (the G/G genotype) after LPS stimulation. Furthermore, mutant microRNAs that bind to the C allele inhibited the luciferase activity of the construct containing the C allele. These results indicate that genetic variation of rs11536889 contributes to translational regulation of TLR4, possibly by binding to microRNAs. | |||||
書誌情報 |
Journal of Biological Chemistry 巻 287, 号 30, p. 25163-25172, 発行日 2012-07-20 |
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出版者 | ||||||
出版者 | American Society for Biochemistry and Molecular Biology Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219258 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1083351X | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1074/jbc.M111.338426 | |||||
権利 | ||||||
権利情報 | This research was originally published in Journal of Biological Chemistry. Sato, K., Yoshimura, A., Kaneko, T., Ukai, T., Ozaki, Y., Nakamura, H., Li, X., Matsumura, H., Hara, Y., Ogata, Y.. A single nucleotide polymorphism in 3′-untranslated region contributes to the regulation of toll-like receptor 4 translation. Journal of Biological Chemistry. 2012; 287(30): pp.25163-25172. © the American Society for Biochemistry and Molecular Biology. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Biological Chemistry, 287(30), pp.25613-25172; 2012 |