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Intrageneric fusions of isolated protoplasts from Ulva and Porphyra by electrofusion method
http://hdl.handle.net/10069/29895
http://hdl.handle.net/10069/29895ccb0da46-fe9f-4f21-a0a9-8f20097c9b5c
名前 / ファイル | ライセンス | アクション |
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suisan68_021.pdf (2.0 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-11-30 | |||||
タイトル | ||||||
タイトル | Intrageneric fusions of isolated protoplasts from Ulva and Porphyra by electrofusion method | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electrofusion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protoplasts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ulva | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porphyra | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Reddy, C.R.K.
× Reddy, C.R.K.× Saito, Munehisa× Migita, Seiji× Fujita, Yuji |
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著者別名 | ||||||
姓名 | レディー, C.R.K | |||||
著者別名 | ||||||
姓名 | 齋藤, 宗久 | |||||
著者別名 | ||||||
姓名 | 右田, 清治 | |||||
著者別名 | ||||||
姓名 | 藤田, 雄二 | |||||
その他のタイトル | ||||||
その他のタイトル | 電気刺激法によるアオサとアマノリのプロトプラストの属内融合 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Isolated algal protoplasts of 1. Ulva pertusa (sterile mutant) and U. conglobata, 2. Porphyra yezoensis normal and P. yezoensis green type, were electrically fused. The protoplasts from each alga were mixed together with its algal partner in a 1:1 ratio in low conductivity electrofusion solution at a density of 1×10⁵⁻⁶ cells/ml. Protoplasts were aligned into short chains in high frequency (1 MHz) alternate current (AC) field and sudsequently fused by the application of a single short duration direct current (DC) pulse. Protoplasts aligned at 200 V for 10 s and 40 V for 20 s yielded maximum pairs about 25 and 40% in Ulva and Porphyra respectively. The application of 20-25 μ s duration DC pulse of 200 V resulted optimum binary fusion percentages about 12% in Ulva and whereas 250 V of 40 μ s duration yielded maximum fusions about 16% in Porphyra. The application of a high intensity DC pulse (> 300 V of 30 μ s duration for Ulva and > 350 V of 40 μ s duration for Porphyra) to the aligned protoplasts induced protoplast lysis. | |||||
書誌情報 |
長崎大学水産学部研究報告 巻 68, p. 21-27, 発行日 1990-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40002770892 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, v.68, pp.21-27; 1990 |