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シオミズツボワムシのプロテアーゼに関する研究-1 : 粗酵素液中のプロティナーゼ活性の性質について
http://hdl.handle.net/10069/30649
http://hdl.handle.net/10069/30649ae3fefa2-11d8-483d-a0e4-998c69636b76
名前 / ファイル | ライセンス | アクション |
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suisan46_031.pdf (384.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-15 | |||||
タイトル | ||||||
タイトル | シオミズツボワムシのプロテアーゼに関する研究-1 : 粗酵素液中のプロティナーゼ活性の性質について | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
原, 研治
× 原, 研治× 石原, 忠× 荒野, 拓× 保田, 正人 |
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著者別名 | ||||||
姓名 | Hara, Kenji | |||||
著者別名 | ||||||
姓名 | Ishihara, Tadashi | |||||
著者別名 | ||||||
姓名 | Arano, Hiraki | |||||
著者別名 | ||||||
姓名 | Yasuda, Masato | |||||
その他のタイトル | ||||||
その他のタイトル | Studies on Protease of the Rotifer, Brachionus plicatilis―I Some Properties of Proteinase in the Crude Extracts | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Acid and alkaline proteinase activities were detected in an ammonium sulfate fraction (50-80%) of crude extracts from the rotifer, Brachionus plicatilis. Some properties of the activities in the fraction were investigated. The optimum pH was obtained at about 4.0 for acid proteinase and at about 8.0 for alkaline one. The optimum temperatures of the acid and the alkaline proteinase were 60 and 50℃, respectively, under the experimental condition. The both proteinase activities were unstable as heated at 60℃ for 10 minutes. The acid proteinase activity was inhibited with 1×10⁻³M of Hg²⁺, Cu²⁺, Ag⁺, I₂, monoiodoacetate and PCMB. The alkaline proteinase activity was inhibited with 1×10⁻³M of Hg²⁺, Cu²⁺, Ag⁺, I₂, NBS, DFP, TLCK and potassium permangate. | |||||
書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries, Nagasaki University 巻 46, p. 31-35, 発行日 1979-02 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40002770671 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, v.46, pp.31-35; 1979 |