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シオミズツボワムシのプロテアーゼに関する研究-2 : 数種の合成基質分解活性の性質について
http://hdl.handle.net/10069/30650
http://hdl.handle.net/10069/30650b926717a-cae7-43eb-863b-4fb8fa83677e
名前 / ファイル | ライセンス | アクション |
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suisan46_037.pdf (367.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-15 | |||||
タイトル | ||||||
タイトル | シオミズツボワムシのプロテアーゼに関する研究-2 : 数種の合成基質分解活性の性質について | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
原, 研治
× 原, 研治× 石原, 忠× 荒野, 拓× 保田, 正人 |
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著者別名 | ||||||
姓名 | Hara, Kenji | |||||
著者別名 | ||||||
姓名 | Ishihara, Tadashi | |||||
著者別名 | ||||||
姓名 | Arano, Hiraki | |||||
著者別名 | ||||||
姓名 | Yasuda, Masato | |||||
その他のタイトル | ||||||
その他のタイトル | Studies on Protease of the Rotifer, Brachionus plicatilis―II Hydrolytic Properties on Some Synthetic Substrates | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Some properties of acid and alkaline proteinase activities in crude extract from the rotifer, Brachionus plicatilis, were reported in the previous paper (1). In the present paper, some properties of hydrolytic activities by the enzyme on CGT, BAA and GPA were investigated. The results of this study were summarized as follows. 1) Two kinds of CGT hydrolytic activity were detected, one was acting in acid pH range (optimum pH was 2.0) and the other was in neutral range (optimum pH was 7.5). The enzyme activity in acid pH range was very unstable and disappeared completely as exposed to room temperature at pH 2.0 for 4 hours. The enzyme activity at pH 7.5 was activated by means of preincubation at 50°~60℃ for 30 minutes, and inhibited with IAA. 2) The optimum pH for BAA hydrolytic activity was about 6 and cysteine was required to activate the enzyme, which was inhibited considerably with Zn²⁺ and IAA, and partially with I₂ and PCMB. 3) GPA hydrolytic activity required cysteine too. The optimum pH was about 6 and the activity was inhibited with Zn²⁺, I₂ and PCMB. | |||||
書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries, Nagasaki University 巻 46, p. 37-42, 発行日 1979-02 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40002770672 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, v.46, pp.37-42; 1979 |