WEKO3
アイテム
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Rubbing Gastric Serosal Surface Enhances Naked Plasmid DNA Transfer in Rats and Mice
http://hdl.handle.net/10069/26171
http://hdl.handle.net/10069/2617147540ed9-5e2b-453b-8524-17e5b8a6c6d3
名前 / ファイル | ライセンス | アクション |
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BPB34_1514.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-10-31 | |||||
タイトル | ||||||
タイトル | Rubbing Gastric Serosal Surface Enhances Naked Plasmid DNA Transfer in Rats and Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Naked plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-viral gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transfection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mine, Toyoharu
× Mine, Toyoharu× Ishii, Hiroki× Nakajima, Sayuri× Yoshikawa, Naoki× Miyamoto, Hirotaka× Nakashima, Mikiro× Nakamura, Junzo× Fumoto, Shintaro× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have developed in vivo gene transfer to mesothelial cells on the peritoneal organs, including the stomach. Simple instillation of naked plasmid DNA onto the gastric serosal surface in mice resulted in effective but transient transgene expression. Here, we developed a simple method to improve not only the transfection efficiency but also the duration of transgene expression. Rubbing the gastric serosal surface using a medical spoon immediately after instillation of naked plasmid DNA onto the gastric serosal surface resulted in 59-fold higher transgene expression 24 h after administration in rats. Without rubbing, transgene expression decreased under the detection limit 7 d after administration. On the other hand, rubbing the gastric serosal surface with a medical spoon after instillation of plasmid DNA prolonged transgene expression for one month. Mechanistic study in mice revealed that improved transfection should not be due to stimulation of cell function such as macropinocytosis by rubbing because rubbing before instillation of plasmid DNA did not improve transfection. Plasmid DNA should enter effectively into cells during rubbing. These findings are valuable to develop an effective method of in vivo gene transfer into peritoneal organs. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 34, 号 9, p. 1514-1517, 発行日 2011-09-01 |
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出版者 | ||||||
出版者 | The Pharmaceutical Society of Japan | |||||
出版者別言語 | ||||||
日本薬学会 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.34.1514 | |||||
権利 | ||||||
権利情報 | (c) 2011 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin, 34(9), pp.1514-1517; 2011 |