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Isolation and characterization of cancer stem-like side population cells in human oral cancer cells.
http://hdl.handle.net/10069/25716
http://hdl.handle.net/10069/257167d92efbe-f4c5-4664-9370-f680bd9adabd
名前 / ファイル | ライセンス | アクション |
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OraOnc47_855.pdf (864.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-09-09 | |||||
タイトル | ||||||
タイトル | Isolation and characterization of cancer stem-like side population cells in human oral cancer cells. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cancer stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemoresistance | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Oral cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Side population | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yanamoto, Souichi
× Yanamoto, Souichi× Kawasaki, Goro× Yamada, Shin-Ichi× Yoshitomi, Izumi× Kawano, Toshihiro× Yonezawa, Hisanobu× Rokutanda, Satoshi× Naruse, Tomofumi× Umeda, Masahiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recent studies suggest that cancer stem cells may be responsible for tumorigenesis and contribute to some individuals' resistance to cancer therapy. Some studies demonstrate that side population (SP) cells isolated from diverse cancer cell lines harbor stem cell-like properties; however, there are few reports examining the role of SP cells in human oral cancer. To determine whether human oral cancer cell lines contain a SP cell fraction, we first isolated SP cells by fluorescence activated cell sorting, followed by culturing in serum-free medium (SFM) using the SCC25 tongue cancer cell line, so that SP cells were able to be propagated to maintain the CSC property. Differential expression profile of stem cell markers (ABCG2, Oct-4 and EpCAM) was examined by RT-PCR in either SP cells or non-SP cells. Growth inhibition by 5-FU was determined by the MTT assay. Clonogenic ability was evaluated by colony formation assay. SCC25 cells contained 0.23% SP cells. The fraction of SP cells was available to grow in SFM cultures. SP cells showed higher mRNA expression of stem cell markers (ABCG2, Oct-4 and EpCAM) as compared with non-SP cells. Moreover, SP cells demonstrated more drug resistance to 5-FU, as compared with non-SP cells. The clone formation efficiency of SP cells was significantly higher than non-SP cells at an equal cell number (P<0.01). We isolated cancer stem-like SP cells from an oral cancer cell line. SP cells possessed the characteristics of cancer stem cells, chemoresistance, and high proliferation ability. Further characterization of cancer stem-like SP cells may provide new insights for novel therapeutic targets. | |||||
書誌情報 |
Oral oncology 巻 47, 号 9, p. 855-860, 発行日 2011-09 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13688375 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10846349 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21757396 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.oraloncology.2011.06.501 | |||||
権利 | ||||||
権利情報 | Copyright © 2011 Elsevier Ltd All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Oral oncology, 47(9), pp.855-860; 2011 |