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Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow
http://hdl.handle.net/10069/22958
http://hdl.handle.net/10069/22958fdc3817f-f2d8-46a9-8ce5-89ce58e45d34
名前 / ファイル | ライセンス | アクション |
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BPB33_107.pdf (646.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-02-24 | |||||
タイトル | ||||||
タイトル | Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Benzalkonium chloride | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Corneal barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Corneal transepithelial electrical resistance | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Electrophysiological method | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mimicking tear flow | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ussing chamber | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakamura, Tadahiro
× Nakamura, Tadahiro× Teshima, Mugen× Kitahara, Takashi× Sasaki, Hitoshi× Uematsu, Masafumi× Kitaoka, Takashi× Nakashima, Mikiro× Nishida, Koyo× Nakamura, Junzo× Higuchi, Shun |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed a new electrophysiological method mimicking tear flow to evaluate the epithelial tight junction of rabbit cornea quantitatively. We investigated the effect of tear flow on the corneal damage induced by ophthalmic preservatives using this method. An Ussing chamber system with Ag/AgCl electrodes was used in the electrophysiological experiment. The excised rabbit cornea was mounted in the Ussing chamber and the precorneal solution in the chamber was perfused with a peristaltic pump at the rate of human tear flow. Corneal transepithelial electrical resistance (TEER) was monitored as corneal barrier ability. In the electrophysiological method mimicking tear flow, we observed stable TEER, which rapidly decreased with benzalkonium chloride (BAC), an eye drop preservative. Using this system, we first found that 0.004% BAC decreased corneal TEER reversibly. A high concentration of BAC showed strong irreversible damage to the tight junction. The influence of BAC on corneal TEER was not only concentration-dependent but also tear flow rate-dependent. The electrophysiological method mimicking tear flow was useful to evaluate the corneal barrier quantitatively. Using this method, we clarified that the tear flow was important to protect the corneal damage induced by preservatives. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 33, 号 1, p. 107-110, 発行日 2010-01 |
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出版者 | ||||||
出版者 | The Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.33.107 | |||||
権利 | ||||||
権利情報 | © 2010 Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin, 33(1), pp.107-110; 2010 |