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A methodology for assessing tumor clonality of adult T-cell leukemia/lymphoma
http://hdl.handle.net/10069/00040746
http://hdl.handle.net/10069/0004074609eebd7c-1926-4006-bcd0-fa871f841833
名前 / ファイル | ライセンス | アクション |
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ISYK1308_Yamakawa.pdf (1.5 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2021-05-11 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A methodology for assessing tumor clonality of adult T-cell leukemia/lymphoma | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
山川, 智弘
× 山川, 智弘 |
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著者別名 | ||||||
姓名 | Yamakawa, Tomohiro | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | While clonal heterogeneity has been demonstrated in most cancers, quantitative assessment of individual tumor clones has not been translated to inform clinical practice. A few methods have been developed to investigate the tumor clonality of adult T cell leukemia/lymphoma (ATLL), but currently there is no clinically translatable method available for quantifying individual tumor clones in ATLL patients. Here, we present a methodology to assess the tumor clonality of ATLL and quantify patient-specific tumor clones in a clinical setting. The methodology consists of three steps: (1) selective amplification of restriction fragments containing a human T cell leukemia virus type 1 (HTLV-1) integration site, (2) amplicon deep sequencing to estimate the clonal structure and identify HTLV-1 integration sites of dominant clones, and (3) digital PCR targeting the HTLV-1 integration sites of the dominant clones to quantify specific tumor clones. We successfully tracked individual tumor clones using this approach and demonstrated that each clone had a distinct response to therapies. The procedure is straightforward and clinically feasible, which should facilitate the proper assessment and management of ATLL. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1308号 学位授与年月日:令和3年3月3日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Tomohiro Yamakawa, Naoki Uno, Daisuke Sasaki, Norihito Kaku, Kei Sakamoto, Kosuke Kosai, Hiroo Hasegawa, Yasushi Miyazaki and Katsunori Yanagihara | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Molecular Therapy - Methods & Clinical Development, 19, pp.467-473; 2020 | |||||
書誌情報 |
Molecular Therapy - Methods & Clinical Development 巻 19, p. 467-473, 発行日 2020 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2329-0501 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.omtm.2020.10.015 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | 成人T細胞白血病/リンパ腫のクローン構成を評価する新規方法の開発 | |||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/00040577 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2021-03-03 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第1308号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2021-03-03) |