A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25?37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.
雑誌名
Fisheries Science
巻
75
号
3
ページ
765 - 770
発行年
2009-05
ISSN
09199268
書誌レコードID
AA10993718
DOI
10.1007/s12562-009-0085-1
権利
c The Japanese Society of Fisheries Science 2009
The original publication is available at www.springerlink.com
Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen
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