Tetrodotoxin (TTX) was intramuscularly administered to non-toxic cultured specimens of the pufferfish Takifugu rubripes to investigate TTX transfer/accumulation profiles in the pufferfish body. In two groups of test fish administered either 50MU/individual of TTX standard (purified TTX; PTTX) or crude extract of toxic pufferfish ovary (crude TTX; CTTX), TTX rapidly transferred from the muscle via the blood to other organs. The toxin transfer profiles differed between groups, however, from 4 to 72h. In the PTTX group, little TTX was retained in the liver, and most (>96%) of the toxin remaining in the body transferred/accumulated in the skin after 12h, whereas in the CTTX group, a considerable amount of toxin (15%-23% of the administered toxin or 28%-58% of the remaining toxin) was transferred/retained in the liver for up to 24h, despite the fact that 89% of the remaining toxin transferred/accumulated in the skin at the end of rearing period (168h). The total amount of toxin remaining in the entire body at 1-4h was approximately 60% of the administered toxin in both groups, which decreased at 8-12h, and then increased again to approximately 60%-80% at 24-168h. Immunohistochemical observation revealed that the toxin accumulated in the skin was localized at the basal cells of the epidermal layer.
雑誌名
Toxicon : official journal of the International Society on Toxinology
巻
53
号
1
ページ
99 - 103
発行年
2009-01
出版者
Elsevier
ISSN
00410101
書誌レコードID
AA11540151
PubMed番号
18996407
DOI
10.1016/j.toxicon.2008.10.018
権利
Copyright c 2008 Elsevier Ltd All rights reserved.
著者版フラグ
author
関係URI
www.elsevier.com/locate/toxicon
引用
Toxicon : official journal of the International Society on Toxinology, 53(1), pp.99-103; 2009
Transfer profile of intramuscularly administered tetrodotoxin to non-toxic cultured specimens of the pufferfish Takifugu rubripes.
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