{"created":"2023-05-15T16:30:03.087278+00:00","id":1037,"links":{},"metadata":{"_buckets":{"deposit":"13204b2f-872b-43d5-b54b-ffd469f5a492"},"_deposit":{"created_by":2,"id":"1037","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1037"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001037","sets":["27:28"]},"author_link":["4931","4936","4933","4932","4930","4935","4934"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e0191099","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bone marrow concentrate (BMC), which is enriched in mononuclear cells (MNCs) and platelets, has recently attracted the attention of clinicians as a new optional means for bone engineering. We previously reported that the osteoinductive effect of bone morphogenetic protein-2 (BMP-2) could be enhanced synergistically by co-transplantation of peripheral blood (PB)-derived platelet-rich plasma (PRP). This study aims to investigate whether BMC can effectively promote bone formation induced by low-dose BMP-2, thereby reducing the undesirable side-effects of BMP-2, compared to PRP. Human BMC was obtained from bone marrow aspirates using an automated blood separator. The BMC was then seeded onto β-TCP granules pre-adsorbed with a suboptimal-dose (minimum concentration to induce bone formation at 2 weeks in mice) of recombinant human (rh) BMP-2. These specimens were transplanted subcutaneously to the dorsal skin of immunodeficient-mice and the induction of ectopic bone formation was assessed 2 and 4 weeks post-transplantation. Transplantations of five other groups [PB, PRP, platelet-poor plasma (PPP), bone marrow aspirate (BM), and BM-PPP] were employed as experimental controls. Then, to clarify the effects on vertical bone augmentation, specimens from the six groups were transplanted for on-lay placement on the craniums of mice. The results indicated that BMC, which contained an approximately 2.5-fold increase in the number of MNCs compared to PRP, could accelerate ectopic bone formation until 2 weeks post-transplantation. On the cranium, the BMC group promoted bone augmentation with a suboptimal-dose of rhBMP-2 compared to other groups. Particularly in the BMC specimens harvested at 4 weeks, we observed newly formed bone surrounding the TCP granules at sites far from the calvarial bone. In conclusion, the addition of BMC could reduce the amount of rhBMP-2 by one-half via its synergistic effect on early-phase osteoinduction. We propose here that BMC transplantation facilitates the clinical use of rhBMP-2 as an alternative strategy for bone engineering.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 13(1), e0191099; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0191099","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2018 Egashira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Egashira, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sumita, Yoshinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhong, Weijian"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"I, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohba, Seigo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagai, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asahina, Izumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"PLoS13_191099.pdf","filesize":[{"value":"28.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS13_191099.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1037/files/PLoS13_191099.pdf"},"version_id":"ceae9b7a-5a02-47fa-bee0-7a3c67f10830"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bone marrow concentrate promotes bone regeneration with a suboptimal-dose of rhBMP-2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bone marrow concentrate promotes bone regeneration with a suboptimal-dose of rhBMP-2"}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-07"},"publish_date":"2018-06-07","publish_status":"0","recid":"1037","relation_version_is_last":true,"title":["Bone marrow concentrate promotes bone regeneration with a suboptimal-dose of rhBMP-2"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:08:44.015173+00:00"}