{"created":"2023-05-15T16:37:13.896276+00:00","id":10550,"links":{},"metadata":{"_buckets":{"deposit":"ca40c96d-db57-4261-b4b2-1c8f8e66131d"},"_deposit":{"created_by":2,"id":"10550","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"10550"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00010550","sets":["73:74"]},"author_link":["41821","41820","41818","41817","41819","41822","41824","41823"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"215","bibliographicPageStart":"210","bibliographicVolumeNumber":"420","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nonsteroidal anti-inflammatory drugs (NSAIDs) are valuable agents; however, their use has been limited by their association with mucosal damage in the upper gastrointestinal tract. NSAIDs inhibit cyclooxygenase and consequently block the synthesis of prostaglandins, which have cytoprotective effects in gastric mucosa; these effects on prostaglandins have been thought to be major cause of NSAID-induced ulceration. However, studies indicate that additional NSAID-related mechanisms are involved in formation of gastric lesions. Here, we used a toxicoproteomic approach to understand cellular processes that are affected by NSAIDs in mouse stomach tissue during ulcer formation. We used fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS)-which consists of fluorogenic derivatization, separation and fluorescence detection by LC, and identification by LC-tandem mass spectrometry-in this proteomic analysis of pyrolic stomach from control and diclofenac (Dic)-treated mice. FD-LC-MS/MS results were highly sensitive; 10 differentially expressed proteins were identified, and all 10 were more highly expressed in Dic-treated mice than in control mice. Specifically, expression levels of 78. kDa glucose-regulated protein (GRP78), heat shock protein beta-1 (HSP27), and gastrin were more than 3-fold higher in Dic-treated mice than in control mice. This study represents a first step to ascertain the precise actors of early NSAID-induced ulceration.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biochemical and Biophysical Research Communications, 420(1), pp.210-215; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2012.03.009","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Elsevier Inc."},{"subitem_rights":"NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, 420, 1(2012)"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiokawa, Akina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Kosei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuyama, Ritsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichibangase, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imai, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"BBRC420_210.pdf","filesize":[{"value":"560.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BBRC420_210.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/10550/files/BBRC420_210.pdf"},"version_id":"45d21ea2-32df-4890-b19a-51a4175b7185"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fluorogenic derivatization-liquid chromatography tandem mass spectrometry","subitem_subject_scheme":"Other"},{"subitem_subject":"Gastric ulceration","subitem_subject_scheme":"Other"},{"subitem_subject":"Nonsteroidal anti-inflammatory drugs","subitem_subject_scheme":"Other"},{"subitem_subject":"Toxicoproteomics","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Toxicoproteomic analysis of a mouse model of nonsteroidal anti-inflammatory drug-induced gastric ulcers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Toxicoproteomic analysis of a mouse model of nonsteroidal anti-inflammatory drug-induced gastric ulcers"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-08-24"},"publish_date":"2012-08-24","publish_status":"0","recid":"10550","relation_version_is_last":true,"title":["Toxicoproteomic analysis of a mouse model of nonsteroidal anti-inflammatory drug-induced gastric ulcers"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:25:49.942670+00:00"}