{"created":"2023-05-15T16:37:19.746315+00:00","id":10688,"links":{},"metadata":{"_buckets":{"deposit":"261c46ec-8200-49bd-8f64-3a6ef8b1f56c"},"_deposit":{"created_by":2,"id":"10688","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"10688"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00010688","sets":["8:9"]},"author_link":["42179","42186","42188","42174","42181","42184","42185","42190","42180","42176","42175","42187","42177","42189","42183","42178","42182"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-05-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"R119","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Arthritis Research & Therapy"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Monosodium urate (MSU) has been shown to promote inflammasome activation and interleukin-1β (IL-1β) secretion in monocyte/macrophages, but the cellular pathway and nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation in synovial tissues, remain elusive. In this study, we investigated the effects of MSU on synovial fibroblasts to elucidate the process of MSU-mediated synovial inflammation.Methods: Human synovial fibroblasts were stimulated with MSU in the presence or absence of serum amyloid A (SAA). The cellular supernatants were analyzed by immunoblotting using anti-IL-1β or anti-caspase-1 antibodies. IL-1β or NLRP3 mRNA expressions were analyzed by real-time PCR or reverse transcription-PCR (RT-PCR) method.Results: Neither SAA nor MSU stimulation resulted in IL-1β or interleukin-1α (IL-1α) secretions and pro-IL-1β processing in synovial fibroblasts. However, in SAA-primed synovial fibroblasts, MSU stimulation resulted in the activation of caspase-1 and production of active IL-1β and IL-1α. The effect of SAA on IL-1β induction was impaired in cells by silencing NLRP3 using siRNA or treating with caspase-1 inhibitor. In addition, SAA induced the secretion of cathepsin B and NLRP3 mRNA expression in synovial fibroblasts.Conclusions: Our data demonstrate that exposure of human synovial fibroblasts to SAA promotes MSU-mediated caspase-1 activation and IL-1β secretion in the absence of microbial stimulation. These findings provide insight into the molecular processes underlying the synovial inflammatory condition of gout.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Arthritis Research & Therapy, 14(3), R119; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/ar3849","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Migita et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons\nAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in\nany medium, provided the original work is properly cited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14786354","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Migita, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koga, Tomohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satomura, Kenshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumi, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Torigoshi, Takafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda, Yumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumi, Yasumori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jiuchi, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyashita, Taiichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamasaki, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aiba, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Komori, Atsumasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Minoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Motokawa, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishibashi, Hiromi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"ART14_R119.pdf","filesize":[{"value":"654.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ART14_R119.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/10688/files/ART14_R119.pdf"},"version_id":"8b3f90aa-0496-4ebe-92ab-89628ad2b7bd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cathepsin B","subitem_subject_scheme":"Other"},{"subitem_subject":"cryopyrin","subitem_subject_scheme":"Other"},{"subitem_subject":"interleukin 1alpha","subitem_subject_scheme":"Other"},{"subitem_subject":"interleukin 1beta","subitem_subject_scheme":"Other"},{"subitem_subject":"messenger RNA","subitem_subject_scheme":"Other"},{"subitem_subject":"serum amyloid A","subitem_subject_scheme":"Other"},{"subitem_subject":"small interfering RNA","subitem_subject_scheme":"Other"},{"subitem_subject":"urate","subitem_subject_scheme":"Other"},{"subitem_subject":"article","subitem_subject_scheme":"Other"},{"subitem_subject":"controlled study","subitem_subject_scheme":"Other"},{"subitem_subject":"fibroblast","subitem_subject_scheme":"Other"},{"subitem_subject":"human","subitem_subject_scheme":"Other"},{"subitem_subject":"human cell","subitem_subject_scheme":"Other"},{"subitem_subject":"human tissue","subitem_subject_scheme":"Other"},{"subitem_subject":"immunoblotting","subitem_subject_scheme":"Other"},{"subitem_subject":"real time polymerase chain reaction","subitem_subject_scheme":"Other"},{"subitem_subject":"reverse transcription polymerase chain reaction","subitem_subject_scheme":"Other"},{"subitem_subject":"synovial fluid","subitem_subject_scheme":"Other"},{"subitem_subject":"synovitis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-09-14"},"publish_date":"2012-09-14","publish_status":"0","recid":"10688","relation_version_is_last":true,"title":["Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:24:45.773774+00:00"}