{"created":"2023-05-15T16:30:09.083238+00:00","id":1174,"links":{},"metadata":{"_buckets":{"deposit":"b7e59612-220b-4135-9f58-c19dfebe9690"},"_deposit":{"created_by":2,"id":"1174","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1174"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001174","sets":["10:11"]},"author_link":["5545","5549","5546","5550","5551","5552","5547","5548","5544"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageStart":"00717-17","bibliographicVolumeNumber":"86","bibliographic_titles":[{"bibliographic_title":"Infection and Immunity"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CD8+ T cells are the major effector cells that protect against malaria liverstage infection, forming clusters around Plasmodium-infected hepatocytes and eliminating parasites after a prolonged interaction with these hepatocytes. We aimed to investigate the roles of specific and nonspecific CD8+ T cells in cluster formation and protective immunity. To this end, we used Plasmodium berghei ANKA expressing ovalbumin as well as CD8+ T cells from transgenic mice expressing a T cell receptor specific for ovalbumin (OT-I) and CD8+ T cells specific for an unrelated antigen, respectively. While antigen-specific CD8+ T cells were essential for cluster formation, both antigen-specific and nonspecific CD8+ T cells joined the clusters. However, nonspecific CD8+ T cells did not significantly contribute to protective immunity. In the livers of infected mice, specific CD8+ T cells expressed high levels of CD25, compatible with a local, activated effector phenotype. In vivo imaging of the liver revealed that specific CD8+ T cells interact with CD11c+ cells around infected hepatocytes. The depletion of CD11c+ cells virtually eliminated the clusters in the liver, leading to a significant decrease in protection. These experiments reveal an essential role of hepatic CD11c+ dendritic cells and presumably macrophages in the formation of CD8+ T cell clusters around Plasmodium-infected hepatocytes. Once cluster formation is triggered by parasite-specific CD8+ T cells, specific and unrelated activated CD8+ T cells join the clusters in a chemokine- and dendritic cell-dependent manner. Nonspecific CD8+ T cells seem to play a limited role in protective immunity against Plasmodium parasites.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Infection and Immunity, 86(4), 00717-17; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/IAI.00717-17","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c American Society for Microbiology, Infection and Immunity, 86(4), 00717-17; 2018."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00199567","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"10985522","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Akbari, Masoud"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Kazumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Bayarsaikhan, Ganchimeg"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyakoda, Mana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Juriasingani, Smriti"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yuda, Masao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Amino, Rogerio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yui, Katsuyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"InfImm86_00717-17.pdf","filesize":[{"value":"673.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"InfImm86_00717-17.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1174/files/InfImm86_00717-17.pdf"},"version_id":"ce013514-aff8-4463-ba10-84e73bf4cfe2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nonspecific CD8+ T Cells and Dendritic Cells/Macrophages Participate in Formation of CD8+ T Cell-Mediated Clusters against Malaria Liver-Stage Infection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nonspecific CD8+ T Cells and Dendritic Cells/Macrophages Participate in Formation of CD8+ T Cell-Mediated Clusters against Malaria Liver-Stage Infection"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-27"},"publish_date":"2018-07-27","publish_status":"0","recid":"1174","relation_version_is_last":true,"title":["Nonspecific CD8+ T Cells and Dendritic Cells/Macrophages Participate in Formation of CD8+ T Cell-Mediated Clusters against Malaria Liver-Stage Infection"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:06:16.038320+00:00"}