{"created":"2023-05-15T16:30:09.171038+00:00","id":1176,"links":{},"metadata":{"_buckets":{"deposit":"1fc2fd1b-4792-445a-be24-c914ec7edbfa"},"_deposit":{"created_by":2,"id":"1176","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1176"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001176","sets":["10:11"]},"author_link":["5561","5564","5563","5562","5565"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"622","bibliographicPageStart":"614","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Metallomics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"As an essential micronutrient, selenium deficiency is a leading cause of cardiovascular diseases. The heart is continuously beating to deliver blood to the entire body, and this requires a high amount of energy. An adult heart normally obtains 50-70% of its adenosine 5′-triphosphate from fatty acid β-oxidation. An increase in fatty acid oxidation activity induces the generation of larger amounts of by-products (reactive oxygen species, ROS) from mitochondrial oxidative phosphorylation. Selenium-dependent glutathione peroxidases play a critical role in the removal of these ROS, especially organic hydroperoxides, from the heart. The definitive transport and/or detailed metabolic pathways from the selenium-source compounds to the selenoproteins in the heart still remain unclear. We explored the selenium-binding proteins in a rat cardiac cell lysate using its reactive metabolic intermediate, selenotrisulfide (STS), and MALDI TOF-mass spectrometry. Several proteins with a free cysteine (Cys) thiol were found to be reactive with STS through a thiol-exchange reaction. The most distinctive Cys-containing protein in the cardiac cell lysate was identified as myoglobin (Mb) from a rat protein database search and tryptic fragmentation experiments. When separately examined in selenium adequate rats, selenium-binding to the cardiac Mb was verified using selenium-specific fluorometry. Cardiac Mb is thought to participate in the selenium metabolic pathway in the heart.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Metallomics, 10(4), pp.614-622; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Royal Society of Chemistry"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1039/C8MT00011E","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c The Royal Society of Chemistry 2018"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"17565901","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1756591X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hori, Eriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Sakura"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuchigami, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haratake, Mamoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Morio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"Metallomics10_614.pdf","filesize":[{"value":"439.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Metallomics10_614.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1176/files/Metallomics10_614.pdf"},"version_id":"011af0b1-f4cc-4a94-a0ee-acb25a29351d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cardiac myoglobin participates in the metabolic pathway of selenium in rats","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cardiac myoglobin participates in the metabolic pathway of selenium in rats"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-05-01"},"publish_date":"2019-05-01","publish_status":"0","recid":"1176","relation_version_is_last":true,"title":["Cardiac myoglobin participates in the metabolic pathway of selenium in rats"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:06:11.653763+00:00"}