{"created":"2023-05-15T16:30:14.696760+00:00","id":1303,"links":{},"metadata":{"_buckets":{"deposit":"d92a53ac-f08d-41db-957e-343dc13eabb1"},"_deposit":{"created_by":2,"id":"1303","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1303"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001303","sets":["73:74"]},"author_link":["6112","6114","6111","6110","6113","6116","6115"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-07-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Suppl 1","bibliographicPageEnd":"94","bibliographicPageStart":"89","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Expert Opinion on Biological Therapy"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives: Prothymosin α (ProTα) was reported to inhibit the neuronal necrosis by facilitating the plasma membrane localization of endocytosed glucose transporter 1/4 through an activation of putative Gi-coupled receptor. The present study aims to identify a novel ProTα target, which may lead to an activation of Gi-coupled receptor. Methods: We used Gi-rich lipid rafts fraction of retinal cell line N18-RE-105 cells for affinity cross-linking. The biological confirmation that F0/F1 ATPase is a target protein complex was performed by cell-free experiments using ELISA-based binding assay, surface plasmon resonance assay and quartz crystal microbalance assay, and cell-based experiments to measure extracellular ATP level in the HUVECs culture. Results: From the cross-linking study and above-mentioned protein-protein interaction assays, ATP5A1 and ATP5B, F1 ATPase subunits were found to ProTα binding target proteins. In the culture of HUVEC cells, furthermore, ProTα increased the extracellular ATP levels in a reversible manner by anti-ATP5A1- and ATP5B-antibodies. Conclusion: The present study suggests that ProTα may activate ecto-F0/F1 ATPase and produced ATP. This study leads to next subjects whether produced ATP and its metabolites, ADP or adenosine may activate corresponding Gi-coupled receptors.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Expert Opinion on Biological Therapy, 18(suppl 1), pp.89-94; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor and Francis Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/14712598.2018.1454427","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Expert Opinion on Biological Therapy on 2018-07-31, available online: http://www.tandfonline.com/.10.1080/14712598.2018.1454427"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14712598","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"17447682","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueda, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsunaga, Hayato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsushita, Yosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda, Shiori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwamoto, Ryusei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokoyama, Shigeyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirouzu, Mikako"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"EOBT18_89.pdf","filesize":[{"value":"551.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"EOBT18_89.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1303/files/EOBT18_89.pdf"},"version_id":"2411ab66-0310-458a-a270-e95b7e5b9d82"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ecto-F0/F1 ATPase","subitem_subject_scheme":"Other"},{"subitem_subject":"HUVEC","subitem_subject_scheme":"Other"},{"subitem_subject":"N18-RE-105 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"protein-protein interaction","subitem_subject_scheme":"Other"},{"subitem_subject":"Prothymosin α","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"1303","relation_version_is_last":true,"title":["Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:03:50.253612+00:00"}