{"created":"2023-05-15T16:30:14.869284+00:00","id":1307,"links":{},"metadata":{"_buckets":{"deposit":"c43bc6ef-050c-41b8-a140-ae6e3cccbb90"},"_deposit":{"created_by":2,"id":"1307","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1307"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001307","sets":["27:28"]},"author_link":["6137","6136"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-08-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"art.no.26","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Skeletal Muscle"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Satellite cells are residential muscle stem cells that express a paired box protein, PAX7. Results: Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7YFP/YFP) were viable, grew and regenerated muscle normally, and Pax7YFP/YFP mouse-derived satellite cells proliferated, differentiated, and self-renewed as efficiently as those from wild-type (Pax7+/+) mice. Conclusions: Taken together, our Pax7-YFP mouse line is a useful tool to aid the development of stem-cell-based therapies for muscle diseases.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Skeletal Muscle, 8(1), art.no.26; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13395-018-0174-x","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2018 The Author(s).This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"20445040","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitajima, Yasuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Yusuke"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"SkeletMus8_26.pdf","filesize":[{"value":"5.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SkeletMus8_26.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1307/files/SkeletMus8_26.pdf"},"version_id":"7434c293-12ba-4d1d-bcad-62ffc53deccc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Knock-in mouse","subitem_subject_scheme":"Other"},{"subitem_subject":"Muscle regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"Myogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"Pax7","subitem_subject_scheme":"Other"},{"subitem_subject":"YFP","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line"}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-09-13"},"publish_date":"2018-09-13","publish_status":"0","recid":"1307","relation_version_is_last":true,"title":["Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:03:46.742995+00:00"}