{"created":"2023-05-15T16:30:14.996131+00:00","id":1310,"links":{},"metadata":{"_buckets":{"deposit":"58d572c5-1455-4d42-a028-c2e4db1bafc8"},"_deposit":{"created_by":6,"id":"1310","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"1310"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001310","sets":["10:22:24"]},"author_link":["116152","116151"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"粉砕された歯石の結晶構造は口腔上皮細胞における細胞死を誘導する"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"361","bibliographicPageStart":"353","bibliographicVolumeNumber":"53","bibliographic_titles":[{"bibliographic_title":"Journal of Periodontal Research"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-20"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17301","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background and Objective: Dental calculus is a mineralized deposit attached to the tooth surface. We have shown that cellular uptake of dental calculus triggers nucleotide‐binding oligomerization domain‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome activation, leading to the processing of the interleukin‐1β precursor into its mature form in mouse and human phagocytes. The activation of the NLRP3 inflammasome also induced a lytic form of programmed cell death, pyroptosis, in these cells. However, the effects of dental calculus on other cell types in periodontal tissue have not been investigated. The aim of this study was to determine whether dental calculus can induce cell death in oral epithelial cells. \nMaterial and Methods: HSC‐2 human oral squamous carcinoma cells, HOMK107 human primary oral epithelial cells and immortalized mouse macrophages were exposed to dental calculus or 1 of its components, hydroxyapatite crystals. For inhibition assays, the cells were exposed to dental calculus in the presence or absence of cytochalasin D (endocytosis inhibitor), z‐YVAD‐fmk (caspase‐1 inhibitor) or glyburide (NLRP3 inflammasome inhibitor). Cytotoxicity was determined by measuring lactate dehydrogenase (LDH) release and staining with propidium iodide. Tumor necrosis factor‐α production was quantified by enzyme‐linked immunosorbent assay. Oral epithelial barrier function was examined by permeability assay. Results: Dental calculus induced cell death in HSC‐2 cells, as judged by LDH release and propidium iodide staining. Dental calculus also induced LDH release from HOMK107 cells. Following heat treatment, dental calculus lost its capacity to induce tumor necrosis factor‐α in mouse macrophages, but could induce LDH release in HSC‐2 cells, indicating a major role of inorganic components in cell death. Hydroxyapatite crystals also induced cell death in both HSC‐2 and HOMK107 cells, as judged by LDH release, indicating the capacity of crystal particles to induce cell death. Cell death induced by dental calculus was significantly inhibited by cytochalasin D, z‐YVAD‐fmk and glyburide, indicating NLRP3 inflammasome involvement. In permeability assays, dental calculus attenuated the barrier function of HSC‐2 cell monolayers. \nConclusion: Dental calculus induces pyroptotic cell death in human oral epithelial cells and the crystalline structure plays a major role in this process. Oral epithelial cell death induced by dental calculus might be important for the etiology of periodontitis.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)甲第1047号 学位授与年月日:平成30年3月20日","subitem_description_type":"Other"},{"subitem_description":"Author: S. M. Ziauddin, A. Yoshimura, J. L. Montenegro Raudales, Y. Ozaki, K. Higuchi, T. Ukai, T. Kaneko, T. Miyazaki, E. Latz, Y. Hara","subitem_description_type":"Other"},{"subitem_description":"Citation: Journal of Periodontal Research, 53(3), pp.353-361; 2018","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(歯学) (2018-03-20)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医歯薬第1047号"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"116152","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ジャウディン, エス　エム"}]}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons Ltd"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/jre.12520","subitem_relation_type_select":"DOI"}}]},"item_5_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10069/38089","subitem_relation_type_select":"HDL"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd"},{"subitem_rights":"This is the peer reviewed version of the following article: Journal of Periodontal Research, 53(3), pp.353-361; 2018, which has been published in final form at http://dx.doi.org/10.1111/jre.12520. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00223484","subitem_source_identifier_type":"ISSN"}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"課程博士"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ziauddin, S. M."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-02"}],"displaytype":"detail","filename":"ISYK1047_Ziauddin.pdf","filesize":[{"value":"3.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYK1047_Ziauddin.pdf","objectType":"fulltext","url":"https://nagasaki-u.repo.nii.ac.jp/record/1310/files/ISYK1047_Ziauddin.pdf"},"version_id":"b5769ac1-80dd-49b0-919e-07db3ea11451"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cell death","subitem_subject_scheme":"Other"},{"subitem_subject":"dental calculus","subitem_subject_scheme":"Other"},{"subitem_subject":"epithelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"inflammasomes","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Crystalline structure of pulverized dental calculus induces cell death in oral epithelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Crystalline structure of pulverized dental calculus induces cell death in oral epithelial cells","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-11-20"},"publish_date":"2018-11-20","publish_status":"0","recid":"1310","relation_version_is_last":true,"title":["Crystalline structure of pulverized dental calculus induces cell death in oral epithelial cells"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:55:55.599391+00:00"}