{"created":"2023-05-15T16:30:15.040763+00:00","id":1311,"links":{},"metadata":{"_buckets":{"deposit":"d7769f3d-91e4-48b2-97b0-c039c1c4508b"},"_deposit":{"created_by":2,"id":"1311","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1311"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001311","sets":["27:28"]},"author_link":["6162","6160","6161","6159","6157","6156","6155","6158"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"516","bibliographicPageStart":"507","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"Microbiology and Immunology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Many members of the phylum Bacteroidetes, such as Flavobacterium johnsoniae, can glide over a solid surface: an ability called gliding motility. It can be usually observed on agar plates as thin, flat, spreading colonies with irregular, feathery edges; this phenomenon is called colony spreading. Colony spreading of F. johnsoniae on 1.5% agar plates containing poor nutrients is dose-dependently inhibited by addition of D-glucose, as previously reported. Accordingly, here, we created mutants (by transposon mutagenesis) that partially suppressed glucose-mediated inhibition of colony spreading. Among the isolates, we found that one had a transposon insertion in Fjoh_4565, tentatively named mfsA, which encodes a major facilitator superfamily (MFS) transporter previously shown to be required for growth on glucose, N-acetyl-glucosamine, and chitin. We constructed an mfsA deletion mutant and found that the mutant showed no glucose-mediated acceleration of growth or glucose uptake. The mfsA gene complemented the phenotype of a glucose-negative Escherichia coli. These results suggest that the mfsA gene encodes the sole MFS transporter of glucose in F. johnsoniae and that glucose uptake is partially required for the glucose-mediated inhibition of F. johnsoniae colony spreading.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Microbiology and Immunology, 62(8), pp.507-516; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons Australia, Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/1348-0421.12633","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2018 The Societies and John Wiley & Sons Australia, Ltd. This is the peer reviewed version of the following article:Microbiology and Immunology, 62(8), pp.507-516; 2018, which has been published in final form at http://dx.doi.org/10.1111/1348-0421.12633 .This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855600","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Imamura, Keigo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Keiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Narita, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Yoshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakane, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naito, Mariko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujiwara, Taku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Koji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"MicImm62_507.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MicImm62_507.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1311/files/MicImm62_507.pdf"},"version_id":"d17a70b2-d709-4e8c-96b4-5eea56e26046"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"colony spreading","subitem_subject_scheme":"Other"},{"subitem_subject":"gliding motility","subitem_subject_scheme":"Other"},{"subitem_subject":"glucose uptake","subitem_subject_scheme":"Other"},{"subitem_subject":"major facilitator superfamily transporter","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake"}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-09-01"},"publish_date":"2019-09-01","publish_status":"0","recid":"1311","relation_version_is_last":true,"title":["Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:03:42.747922+00:00"}