{"created":"2023-05-15T16:39:26.294168+00:00","id":13338,"links":{},"metadata":{"_buckets":{"deposit":"97f16ea9-01d8-4e9b-a5d7-8d923741ed47"},"_deposit":{"created_by":2,"id":"13338","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13338"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013338","sets":["29:30:1713167819629:1123"]},"author_link":["48122","48123","48120","48121","48124","48125"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Acta Medica Nagasakiensia"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is challenging to demonstrate the capsule of Streptococcus pneumoniae (S. pneumoniae) under immunoelectron microscopy because of the thick mucopeptide cell wall hampering proper fixation. A novel rapid freeze fixation method was established to observe the capsule of S. pneumoniae. A strain of serotype 3 of S. pneumoniae isolate was analyzed after rapid freezing. An ethanol freezing-substitution fixing method was applied and immunohistochemical staining with osmium tetroxide was tested. The capsule was confirmed using the serotype 3 specific polyclonal antibodies labeled with colloidal gold particles. To the best of our knowledge, this is the first report of S. pneumoniae capsule by immunoelectron microscope.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Acta Medica Nagasakiensia, 56(1), pp.1-4; 2011","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagasaki University School of Medicine"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508430","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00016055","subitem_source_identifier_type":"ISSN"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"長崎大学医学部"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ichinose, Akitoyo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Kiwao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Senba, Masachika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ahmed, Kamruddin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ariyoshi, Koya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Keizo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"ActMed56_01.pdf","filesize":[{"value":"98.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ActMed56_01.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13338/files/ActMed56_01.pdf"},"version_id":"9af53a15-75f0-4b5e-a7c5-62391fa160e6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pneumococcal capsule","subitem_subject_scheme":"Other"},{"subitem_subject":"rapid freeze-substitution fixation method","subitem_subject_scheme":"Other"},{"subitem_subject":"colloidal gold","subitem_subject_scheme":"Other"},{"subitem_subject":"electron microscopy","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Demonstration of pneumococcal capsule under immunoelectron microscopy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Demonstration of pneumococcal capsule under immunoelectron microscopy"}]},"item_type_id":"3","owner":"2","path":["1123"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-07-22"},"publish_date":"2011-07-22","publish_status":"0","recid":"13338","relation_version_is_last":true,"title":["Demonstration of pneumococcal capsule under immunoelectron microscopy"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2024-04-16T05:31:39.883495+00:00"}