{"created":"2023-05-15T16:39:28.685908+00:00","id":13390,"links":{},"metadata":{"_buckets":{"deposit":"4fbdba2f-40b9-4909-b08b-3f3168fa3e1b"},"_deposit":{"created_by":2,"id":"13390","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13390"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013390","sets":["73:74"]},"author_link":["48369","48370","48371","48368","48367"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"24","bibliographicPageEnd":"3762","bibliographicPageStart":"3757","bibliographicVolumeNumber":"1218","bibliographic_titles":[{"bibliographic_title":"Journal of Chromatography. A"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A method for sequence analysis and identification of phosphoamino acids in peptides based on high performance liquid chromatography (HPLC) is described. The peptides were derivatized with an Edman type reagent, 4-(1'-cyanoisoindolyl)phenylisothiocyanate (CIPIC) and subsequently cleaved to generate stable and fluorescent 4-(1'-cyanoisoindolyl)phenylthiazolinone (CIP-TZ)-amino acids. Several experimental factors that affected derivatization on membranes were examined. Under the optimized conditions, the CIP-TZ derivatives of Try(p), Thr(p) and Ser(p) were obtained and separated from their parent amino acids with baseline resolution using an isocratic elution system. Up to the 4th residue of phosphorylated pentapeptides was successfully identified, whereas phosphoamino acid residues could not be detected by the conventional procedure using phenylisothiocyanate (PITC). The results demonstrated the potential of CIPIC as a derivatization reagent for peptide sequencing and the applicability of the method for the study and identification of phosphoamino acids in peptides.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Chromatography. A, 1218(24), pp.3757-3762; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21531425","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.chroma.2011.04.040","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011 Elsevier B.V. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10985833","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00219673","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18733778","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shibata, Takayuki"}],"nameIdentifiers":[{"nameIdentifier":"48367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wainaina, Moses N"}],"nameIdentifiers":[{"nameIdentifier":"48368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyoshi, Takayuki"}],"nameIdentifiers":[{"nameIdentifier":"48369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kabashima, Tsutomu"}],"nameIdentifiers":[{"nameIdentifier":"48370","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kai, Masaaki"}],"nameIdentifiers":[{"nameIdentifier":"48371","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"JCA1218_3757.pdf","filesize":[{"value":"382.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JCA1218_3757.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13390/files/JCA1218_3757.pdf"},"version_id":"5faa2189-29b5-4ca1-85d4-92cdac63b3e9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CIPIC","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescence detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphorylated peptides","subitem_subject_scheme":"Other"},{"subitem_subject":"Sequencing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenylisothiocyanate.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenylisothiocyanate."}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-08-02"},"publish_date":"2011-08-02","publish_status":"0","recid":"13390","relation_version_is_last":true,"title":["A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenylisothiocyanate."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:52:06.535081+00:00"}