{"created":"2023-05-15T16:39:31.664119+00:00","id":13450,"links":{},"metadata":{"_buckets":{"deposit":"b04d34b3-28ad-4931-b6cb-fbc82d2e4aff"},"_deposit":{"created_by":2,"id":"13450","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13450"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013450","sets":["73:74"]},"author_link":["48642","48644","48645","48641","48643"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"712","bibliographicPageStart":"701","bibliographicVolumeNumber":"149","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Serine dehydrogenase from Escherichia coli is a homotetrameric enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family. This enzyme catalyses the NADP(+)-dependent oxidation of serine to 2-aminomalonate semialdehyde. The enzyme shows a stereospecificity for β-(3S)-hydroxy acid as a substrate; however, no stereospecificity was observed at the α-carbon. The structures of the ligand-free SerDH and SerDH-NADP(+)-phosphate complex were determined at 1.9 and 2.7 Å resolutions, respectively. The overall structure, including the catalytic tetrad of Asn106, Ser134, Tyr147 and Lys151, shows obvious relationships with other members of the SDR family. The structure of the substrate-binding loop and that of the C-terminal region were disordered in the ligand-free enzyme, whereas these structures were clearly defined in the SerDH-NADP(+) complex as a closed form. Interestingly, the C-terminal region was protruded from the main body and it formed an anti-parallel β-sheet with another C-terminal region on the subunit that is diagonally opposite to that in the tetramer. It is revealed that the C-terminal region possesses the important roles in substrate binding through the stabilization of the substrate-binding loop in the closed form complex. The roles of the C-terminal region along with those of the residues involved in substrate recognition were studied by site-directed mutagenesis.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Biochemistry, 149(6), pp.701-712; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21349860","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvr024","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Authors 2011. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved."},{"subitem_rights":"This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Biochemistry following peer review. The definitive publisher-authenticated version Journal of Biochemistry, 149(6), pp.701-712; 2011 is available online at: http://dx.doi.org/10.1093/jb/mvr024."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021924X","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"17562651","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamazawa, Ryuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakajima, Yoshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mushiake, Karin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimoto, Tadashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Kiyoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"JouBio149_701.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JouBio149_701.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13450/files/JouBio149_701.pdf"},"version_id":"62897ee8-99e1-4441-aa10-8fce645373ee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"conformational change","subitem_subject_scheme":"Other"},{"subitem_subject":"crystal structure","subitem_subject_scheme":"Other"},{"subitem_subject":"serine dehydrogenase","subitem_subject_scheme":"Other"},{"subitem_subject":"short-chain dehydrogenase/reductase family","subitem_subject_scheme":"Other"},{"subitem_subject":"substrate recognition","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Crystal structure of serine dehydrogenase from Escherichia coli: important role of the C-terminal region for closed-complex formation.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Crystal structure of serine dehydrogenase from Escherichia coli: important role of the C-terminal region for closed-complex formation."}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-01"},"publish_date":"2012-07-01","publish_status":"0","recid":"13450","relation_version_is_last":true,"title":["Crystal structure of serine dehydrogenase from Escherichia coli: important role of the C-terminal region for closed-complex formation."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:03:05.852735+00:00"}