{"created":"2023-05-15T16:39:36.502767+00:00","id":13564,"links":{},"metadata":{"_buckets":{"deposit":"8c3a0f99-f433-4723-89f7-bf54f76da487"},"_deposit":{"created_by":2,"id":"13564","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13564"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013564","sets":["35:36"]},"author_link":["48974","48976","48977","48975","48978"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"320","bibliographicPageStart":"313","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"Parasitology International"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"SURFIN(4.2) is a parasite-infected red blood cell (iRBC) surface associated protein of Plasmodium falciparum. To analyze the region responsible for the intracellular trafficking of SURFIN(4.2) to the iRBC and Maurer's clefts, a panel of transgenic parasite lines expressing recombinant SURFIN(4.2) fused with green fluorescent protein was generated and evaluated for their localization. We found that the cytoplasmic region containing a tryptophan rich (WR) domain is not necessary for trafficking, whereas the transmembrane (TM) region was. Two PEXEL-like sequences were shown not to be responsible for the trafficking of SURFIN(4.2), demonstrating that the protein is trafficked in a PEXEL-independent manner. N-terminal replacement, deletion of the cysteine-rich domain or the variable region also did not prevent the protein from localizing at the iRBC or Maurer's clefts. A recombinant SURFIN(4.2) protein possessing 50 amino acids upstream of the TM region, TM region itself and a part of the cytoplasmic region was shown to be trafficked into the iRBC and Maurer's clefts, suggesting that there are no essential trafficking motifs in the SURFIN(4.2) extracellular region. A mini-SURFIN(4.2) protein containing WR domain was shown by Western blotting to be more abundantly detected in a Triton X-100-insoluble fraction, compared to the one without WR domain. We suggest that the cytoplasmic region containing the WR may be responsible for their difference in solubility.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Parasitology International, 60(3), pp.313-320; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier BV"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21616162","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.parint.2011.05.003","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011 Elsevier Ireland Ltd All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11112001","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13835769","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18730329","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Alexandre, Jean Semé Fils"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yahata, Kazuhide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawai, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Torii, Motomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Osamu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"ParInt60_313.pdf","filesize":[{"value":"617.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ParInt60_313.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13564/files/ParInt60_313.pdf"},"version_id":"b37c6698-7a60-4ab0-bd99-7392d3c09a76"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Malaria","subitem_subject_scheme":"Other"},{"subitem_subject":"Maurer's clefts","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmodium falciparum","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein trafficking","subitem_subject_scheme":"Other"},{"subitem_subject":"SURFIN","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"PEXEL-independent trafficking of Plasmodium falciparum SURFIN4.2 to the parasite-infected red blood cell and Maurer's clefts.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PEXEL-independent trafficking of Plasmodium falciparum SURFIN4.2 to the parasite-infected red blood cell and Maurer's clefts."}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-08-31"},"publish_date":"2011-08-31","publish_status":"0","recid":"13564","relation_version_is_last":true,"title":["PEXEL-independent trafficking of Plasmodium falciparum SURFIN4.2 to the parasite-infected red blood cell and Maurer's clefts."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:51:02.316021+00:00"}