{"created":"2023-05-15T16:39:43.560399+00:00","id":13725,"links":{},"metadata":{"_buckets":{"deposit":"729ce4d0-0402-4a83-b0b3-15344f35190d"},"_deposit":{"created_by":2,"id":"13725","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13725"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013725","sets":["27:28"]},"author_link":["49356","49357","49355","49359","49354","49353","49358"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-11-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"44","bibliographicPageEnd":"38127","bibliographicPageStart":"38115","bibliographicVolumeNumber":"286","bibliographic_titles":[{"bibliographic_title":"The Journal of Biological Chemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Porphyromonas gingivalis and Porphyromonas endodontalis, asaccharolytic black-pigmented anaerobes, are predominant pathogens of human chronic and periapical periodontitis, respectively. They incorporate di- and tripeptides from the environment as carbon and energy sources. In the present study, we cloned a novel dipeptidyl peptidase (DPP) gene of P. endodontalis ATCC 35406, designated as DPP11. The DPP11 gene encoded 717 amino acids with a molecular mass of 81,090, and was present as a 75-kDa form with an N-terminus of Asp22. A homology search revealed the presence of a P. gingivalis orthologue, PGN0607, which has been categorized as an isoform of authentic DPP7. P. gingivalis DPP11 was exclusively cell-associated as a truncated 60-kDa form and the gene ablation retarded cell growth. DPP11 specifically removed dipeptides from oligopeptides with the penultimate N-terminal Asp and Glu, and has a P2-position preference to hydrophobic residues. Optimum pH was 7.0 and the kcat/Km value was higher for Asp than Glu. Those activities were lost by substitution of Ser652 in P. endodontalis and Ser655 in P. gingivalis DPP11 to Ala, and they were consistently decreased with increasing NaCl concentration. Arg670 is a unique amino acid completely conserved in all DPP11 members distributed in the genera Porphyromonas, Bacteroides, and Parabacteroides, whilst this residue is converted to Gly in all authentic DPP7 members. Substitution analysis suggested that Arg670 interacts with an acidic residue of the substrate. Considered to preferentially utilize acidic amino acids, DPP11 ensures efficient degradation of oligopeptide substrates in these gram-negative anaerobic rods.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"The Journal of Biological Chemistry, 286(44), pp.38115-38127; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21896480","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M111.278572","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011, The American Society for Biochemistry and Molecular Biology"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00251083","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00219258","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1083351X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohara-Nemoto, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimoyama, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Shigenobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kon, Asako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haraga, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nemoto, Takayuki K"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"JBC286_38115.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JBC286_38115.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13725/files/JBC286_38115.pdf"},"version_id":"f1d1dabb-d5eb-45aa-8e25-28d4bed659a0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ASP- and GLU-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ASP- and GLU-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources."}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-09-20"},"publish_date":"2011-09-20","publish_status":"0","recid":"13725","relation_version_is_last":true,"title":["ASP- and GLU-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:09:43.295360+00:00"}