{"created":"2023-05-15T16:39:45.500671+00:00","id":13771,"links":{},"metadata":{"_buckets":{"deposit":"3cf10bbb-c3f5-4fcf-93ff-c75eba6bbd7f"},"_deposit":{"created_by":2,"id":"13771","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13771"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013771","sets":["10:11"]},"author_link":["49478","49477","49482","49479","49481","49480"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"308","bibliographicPageStart":"304","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Japanese Journal of Infectious Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Staphylococcus epidermidis is a cause of orthopedic device-related infection, and to treat such infection, biofilms should be controlled. Polysaccharide intercellular adhesin (PIA) is associated with the biofilm-forming ability of staphylococcal strains. PIA in biofilm-positive staphylococcal strains can be detected by the Congo red agar (CRA) method. In this study, we used the CRA method to examine the effects of subminimal inhibitory concentrations (sub-MICs) of 11 antibacterial agents on PIA production by S. epidermidis. We found that the PIA-negative variants were selected only by sub- MICs of gentamicin (GM). This PIA-negative phenotype was maintained over several generations in the absence of GM. Such selection occurred in six of eight clinical isolates, as well as in the biofilm-positive control strain. No such selection occurred with aminoglycoside antibiotics except for GM. Most of the PIA-negative variants that were selected by GM showed a markedly lower biofilm-forming ability on stainless steel washers than their untreated parent strains. In conclusion, variants with lower biofilmforming ability may be selected by a sub-MIC of GM. Investigation of the reason why variants with reduced biofilm-forming ability can be selected in the presence of sub-MICs of GM may contribute to strategies against biofilm-related infections.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Japanese Journal of Infectious Diseases, 64(4), pp.304-308; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Institute of Infectious Diseases"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1132885X","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13446304","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"国立感染症研究所"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tamai, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsurumoto, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kajiyama, Shiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Adachi, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakimura, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shindo, Hiroyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"JID64_304.pdf","filesize":[{"value":"243.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JID64_304.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13771/files/JID64_304.pdf"},"version_id":"1b9d89d6-f47a-4df6-8619-ca411766cc8e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biofilm deficiency in polysaccharide intercellular adhesin-negative variants of staphylococcus epidermidis selected by subminimal inhibitory concentrations of gentamicin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biofilm deficiency in polysaccharide intercellular adhesin-negative variants of staphylococcus epidermidis selected by subminimal inhibitory concentrations of gentamicin"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-09-26"},"publish_date":"2011-09-26","publish_status":"0","recid":"13771","relation_version_is_last":true,"title":["Biofilm deficiency in polysaccharide intercellular adhesin-negative variants of staphylococcus epidermidis selected by subminimal inhibitory concentrations of gentamicin"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:49:19.973428+00:00"}