{"created":"2023-05-15T16:39:51.653286+00:00","id":13900,"links":{},"metadata":{"_buckets":{"deposit":"2823e153-3b3b-442e-9bf5-8817475d11a1"},"_deposit":{"created_by":2,"id":"13900","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"13900"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00013900","sets":["73:74"]},"author_link":["49837","49843","49847","49836","49844","49842","49845","49846","49839","49834","49841","49838","49840","49835"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageStart":"e25788","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background:Alzheimer's disease (AD) is a neurodegenerative disorder that causes progressive memory and cognitive decline during middle to late adult life. The AD brain is characterized by deposition of amyloid β peptide (Aβ), which is produced from amyloid precursor protein by β- and γ-secretase (presenilin complex)-mediated sequential cleavage. Induced pluripotent stem (iPS) cells potentially provide an opportunity to generate a human cell-based model of AD that would be crucial for drug discovery as well as for investigating mechanisms of the disease. Methodology/Principal Findings:We differentiated human iPS (hiPS) cells into neuronal cells expressing the forebrain marker, Foxg1, and the neocortical markers, Cux1, Satb2, Ctip2, and Tbr1. The iPS cell-derived neuronal cells also expressed amyloid precursor protein, β-secretase, and γ-secretase components, and were capable of secreting Aβ into the conditioned media. Aβ production was inhibited by β-secretase inhibitor, γ-secretase inhibitor (GSI), and an NSAID; however, there were different susceptibilities to all three drugs between early and late differentiation stages. At the early differentiation stage, GSI treatment caused a fast increase at lower dose (Aβ surge) and drastic decline of Aβ production. Conclusions/Significance:These results indicate that the hiPS cell-derived neuronal cells express functional β- and γ-secretases involved in Aβ production; however, anti-Aβ drug screening using these hiPS cell-derived neuronal cells requires sufficient neuronal differentiation.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 6(9), e25788; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLoS ONE"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0025788","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 Yahata et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yahata, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asai, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitaoka, Shiho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Kazutoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asaka, Isao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hioki, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maruyama, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saido, Takaomi C."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakahata, Tatsutoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asada, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamanaka, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwata, Nobuhisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Haruhisa"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"journal.pone.0025788.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"journal.pone.0025788.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/13900/files/journal.pone.0025788.pdf"},"version_id":"c5803e89-b917-493a-9f64-04f2d772963b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-Aβ Drug Screening Platform Using Human iPS Cell-Derived Neurons for the Treatment of Alzheimer's Disease","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-Aβ Drug Screening Platform Using Human iPS Cell-Derived Neurons for the Treatment of Alzheimer's Disease"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-10-11"},"publish_date":"2011-10-11","publish_status":"0","recid":"13900","relation_version_is_last":true,"title":["Anti-Aβ Drug Screening Platform Using Human iPS Cell-Derived Neurons for the Treatment of Alzheimer's Disease"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T00:29:26.841058+00:00"}