{"created":"2023-05-15T16:30:20.269177+00:00","id":1425,"links":{},"metadata":{"_buckets":{"deposit":"e2875f92-769f-4b3b-9b80-82829dd0b2a6"},"_deposit":{"created_by":2,"id":"1425","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1425"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001425","sets":["73:74"]},"author_link":["6721","6722","6725","6723","6724"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1188","bibliographicPageStart":"1183","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"Analytical Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aryl halides are a very important category of compounds that include many vital drugs and key industrial additives, such as clofibrate and bromobenzene, respectively. Due to their importance, our research group previously developed a novel fluorescence labeling approach for their analysis using a fluorescent aryl boronic acid as a reagent, based on the Suzuki coupling reaction. This coupling reaction was successfully applied for the determination of aryl halides in biological fluids; however, there was a limitation of low reactivity towards ortho-substituted aryl halides. In the present study, a novel fluorescence derivatization approach for aryl halides was developed using, 2-(4-ethynylphenyl)-4,5-diphenyl-1Himidazole (DIB-ET) as a fluorescent alkyne reagent, based on the Sonogashira coupling reaction. DIB-ET reacted with aryl bromides in the presence of palladium and copper as catalysts, yielding fluorescent derivatives that could be subsequently determined by an HPLC system with fluorescence detection. The detection limits (S/N = 3) for aryl bromides were in the range of 14 - 23 nM, which is 3.5 - 18-times more sensitive than our previously developed approach, Suzuki coupling derivatization. Moreover, in contrast to the previous technique, the reactivity of DIB-ET to orthosubstituted aryl bromides was almost equivalent to that of the para-substituted aryl bromides. Hence, by using this newly developed approach we could label the aryl halides with more sensitivity and reactivity. Finally, the proposed method was successfully applied for the selective determination of aryl bromides in human serum with good recovery (84.6 - 107%), which proves the ability of the developed method to determine occupational exposure to aryl halides.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Analytical sciences, 34(10), pp.1183-1188; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2116/analsci.18P117","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"2018 cThe Japan Society for Analytical Chemstry"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09106340","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13482246","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japan Society for Analytical Chemistry"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YASAKA, Naoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KISHIKAWA, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIGASHIJIMA, Takumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHYAMA, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KURODA, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"AnaSci34_1183.pdf","filesize":[{"value":"486.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AnaSci34_1183.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1425/files/AnaSci34_1183.pdf"},"version_id":"83eba50f-96bc-4bb8-8d1a-111f7e9e4951"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fluorescence derivatization","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescent alkyne","subitem_subject_scheme":"Other"},{"subitem_subject":"Ortho-substituted aryl halide","subitem_subject_scheme":"Other"},{"subitem_subject":"Sonogashira coupling reaction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Utility of Sonogashira Coupling Reaction for the Derivatization of Aryl Halides with Fluorescent Alkyne","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Utility of Sonogashira Coupling Reaction for the Derivatization of Aryl Halides with Fluorescent Alkyne"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-13"},"publish_date":"2018-11-13","publish_status":"0","recid":"1425","relation_version_is_last":true,"title":["The Utility of Sonogashira Coupling Reaction for the Derivatization of Aryl Halides with Fluorescent Alkyne"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:02:42.704699+00:00"}