@article{oai:nagasaki-u.repo.nii.ac.jp:00000143,
 author = {Mpingabo, Patrick I. and Urata, Shuzo and Yasuda, Jiro},
 journal = {Frontiers in Microbiology},
 month = {Sep},
 note = {Several arenaviruses are highly pathogenic to humans, causing hemorrhagic fever. Discovery of anti-arenavirus drug 
candidates is urgently needed, although the molecular basis of the host- and organ-specific pathogenicity remains to be fully elucidated. The arenavirus Z protein facilitates production of virus-like particles (VLPs), providing an established method to assess virus budding. In this study, we examined the efficiency of VLP production by solely expressing Z protein of several different arenaviruses. In addition, we analyzed the role of the late (L)-domain of the arenavirus Z protein, which is essential for the interaction with ESCRT proteins, in VLP production among different 
cell lines. VLP assay was performed using Z proteins of Junin virus (JUNV), Machupo virus (MACV), Tacaribe virus (TCRV), 
Latino virus (LATV), Pichinde virus (PICV), and Lassa virus (LASV) in six different cell lines: HEK293T, Huh-7, A549, Vero76, BHK-21, and NIH3T3 cells. JUNV, MACV, and LASV Z proteins efficiently produced VLPs in all tested cell lines, while the efficiencies of VLP production by the other arenavirus Z proteins were cell type-dependent. The contribution of the L-domain(s) within Z protein to VLP production also highly depended on the cell type. These results suggested that each arenavirus has its own particle-production mechanism, which is different among the cell types., Frontiers in Microbiology, 11, art.no.562814; 2020},
 title = {Analysis of the Cell Type-Dependence on the Arenavirus Z-Mediated Virus-Like Particle Production},
 volume = {11},
 year = {2020}
}