@article{oai:nagasaki-u.repo.nii.ac.jp:00014522,
 author = {Higa, Yukiko and Toma, Takako and Tsuda, Yoshio and Miyagi, Ichiro},
 issue = {5},
 journal = {Japanese Journal of Infectious Diseases},
 month = {Sep},
 note = {Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species-Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis-found in the Ryukyu Archipelago, Japan. Each newly designed primer was able to amplify a species-specific fragment with a different size. Conditions for multiplex PCR were optimized to identify all five species in a single PCR. This method is a convenient tool for entomological field surveys, particularly in arbovirus endemic/epidemic areas where some of these species coexist., Japanese Journal of Infectious Diseases, 63(5), pp.312-316; 2010},
 pages = {312--316},
 title = {A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan},
 volume = {63},
 year = {2010}
}