@article{oai:nagasaki-u.repo.nii.ac.jp:00014593, author = {Helegbe, Gideon K and Huy, Nguyen T and Yanagi, Tetsuo and Shuaibu, Mohammed N and Kikuchi, Mihoko and Yasunami, Michio and Hirayama, Kenji}, issue = {4}, journal = {Tropical Medicine and Health}, month = {Jan}, note = {A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p‹0.0001. This result correlates significantly with the standard ELISA assay, p‹0.0001, r2=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p‹0.006 (r2=0.33) (fluorometry method) and p‹0.0001, r2=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives., Tropical Medicine and Health, 38(4), pp.127-131; 2011}, pages = {127--131}, title = {Microfluorometric assay for quantification of anti-erythrocytic antibody level in sera of Plasmodium berghei ANKA infected semi-immune mice}, volume = {38}, year = {2011} }