{"created":"2023-05-15T16:30:22.016365+00:00","id":1465,"links":{},"metadata":{"_buckets":{"deposit":"45221222-e1c1-42fd-a9fe-3873036e097c"},"_deposit":{"created_by":2,"id":"1465","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1465"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001465","sets":["10:11"]},"author_link":["6975","6978","6971","6974","6977","6976","6970","6972","6973"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"36","bibliographicPageStart":"34","bibliographicVolumeNumber":"155","bibliographic_titles":[{"bibliographic_title":"Journal of Microbiological Methods"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nontuberculous mycobacteria (NTM) can carry two or more 16S rRNA gene copies that are, in some instances, non-identical. In this study, we used a combined cloning and sequencing approach to analyze 16S rRNA gene sequences of six NTM species, \nMycobacterium cosmeticum, M. pallens, M. hodleri, M. crocinum, M. flavescens, and M. xenopi. Our approach facilitated the identification of two distinct gene copies in each species. The two M. cosmeticum genes had a single nucleotide difference, whereas two nucleotide polymorphisms were identified in M. hodleri, M. flavescens, and M. xenopi. M. pallens had a difference in four nucleotides and M. crocinum ? in 23 nucleotides. \nThus, we showed that the six NTM species possess at least two non-identical 16S rRNA gene copies. The full-length sequences of the intraspecies 16S rRNA variants will facilitate NTM identification and sequence analysis of specimens or other samples.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Microbiological Methods, 155, pp.34-36; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.mimet.2018.08.012","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2018, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01677012","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeda, Keita"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chikamatsu, Kinuyo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Yuriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morishige, Yuta"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murase, Yoshiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aono, Akio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaki, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitarai, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"JMM155_34.pdf","filesize":[{"value":"301.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JMM155_34.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1465/files/JMM155_34.pdf"},"version_id":"b48dd462-2c3e-4fb5-bdba-adcd75017492"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"16S rRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene copy","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycobacterium","subitem_subject_scheme":"Other"},{"subitem_subject":"Nontuberculous mycobacteria","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Six species of nontuberculous mycobacteria carry non-identical 16S rRNA gene copies","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Six species of nontuberculous mycobacteria carry non-identical 16S rRNA gene copies"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-01-01"},"publish_date":"2020-01-01","publish_status":"0","recid":"1465","relation_version_is_last":true,"title":["Six species of nontuberculous mycobacteria carry non-identical 16S rRNA gene copies"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:01:54.047356+00:00"}