{"created":"2023-05-15T16:40:34.133416+00:00","id":14882,"links":{},"metadata":{"_buckets":{"deposit":"4765c980-8391-4183-bf02-075e1441363c"},"_deposit":{"created_by":2,"id":"14882","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"14882"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00014882","sets":["50:51"]},"author_link":["54594","54596","54595","54598","54597"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"296","bibliographicPageStart":"289","bibliographicVolumeNumber":"158","bibliographic_titles":[{"bibliographic_title":"Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64bp 5'-non-coding region, a 675bp open reading frame encoding 225 amino acids, and a 465bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 158(4), pp.289-296; 2011","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21199678","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.cbpb.2010.12.007","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011 Elsevier Inc. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11045017","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10964959","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18791107","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wang, Yajun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Osatomi, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagatomo, Yoshimi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Asami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hara, Kenji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"CBPb158_289.pdf","filesize":[{"value":"283.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CBPb158_289.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/14882/files/CBPb158_289.pdf"},"version_id":"7a5ae244-7a11-4ab6-b50c-6599c3cf3bbc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Amino acid sequence","subitem_subject_scheme":"Other"},{"subitem_subject":"CDNA sequence","subitem_subject_scheme":"Other"},{"subitem_subject":"Hepatopancreas","subitem_subject_scheme":"Other"},{"subitem_subject":"Japanese flounder","subitem_subject_scheme":"Other"},{"subitem_subject":"Manganese-superoxide dismutase","subitem_subject_scheme":"Other"},{"subitem_subject":"Mitochondria-targeting signal","subitem_subject_scheme":"Other"},{"subitem_subject":"Purification","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus).","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)."}]},"item_type_id":"2","owner":"2","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-04-15"},"publish_date":"2011-04-15","publish_status":"0","recid":"14882","relation_version_is_last":true,"title":["Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:51:22.782456+00:00"}