@article{oai:nagasaki-u.repo.nii.ac.jp:00014954,
 author = {Atarashi, Ryuichiro and Satoh, Katsuya and Sano, Kazunori and Fuse, Takayuki and Yamaguchi, Naohiro and Ishibashi, Daisuke and Matsubara, Takehiro and Nakagaki, Takehiro and Yamanaka, Hitoki and Shirabe, Susumu and Yamada, Masahito and Mizusawa, Hidehiro and Kitamoto, Tetsuyuki and Klug, Genevieve and McGlade, Amelia and Collins, Steven J and Nishida, Noriyuki},
 issue = {2},
 journal = {Nature Medicine},
 month = {Feb},
 note = {The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD., Nature Medicine, 17(2), pp.175-178; 2011},
 pages = {175--178},
 title = {Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.},
 volume = {17},
 year = {2011}
}