{"created":"2023-05-15T16:40:47.504569+00:00","id":15191,"links":{},"metadata":{"_buckets":{"deposit":"b35662cb-8520-4a75-9b41-f01422828fbf"},"_deposit":{"created_by":2,"id":"15191","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15191"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015191","sets":["25:26"]},"author_link":["55859","55862","55861","55860"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-05-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"516","bibliographicPageStart":"506","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"DNA repair"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nucleotide excision repair (NER) removes the major UV-photolesions from cellular DNA. In humans, compromised NER activity is the cause of several photosensitive diseases, one of which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays commonly used in measurement of NER activity are 'unscheduled DNA synthesis (UDS)', and 'recovery of RNA synthesis (RRS)', the latter being a specific measure of the transcription-coupled repair sub-pathway of NER. Both assays are key techniques for research in NER as well as in diagnoses of NER-related disorders. Until very recently, reliable methods for these assays involved measurements of incorporation of radio-labeled nucleosides. We have established non-radioactive procedures for determining UDS and RRS levels by incorporation of recently developed alkyne-conjugated nucleoside analogues, 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyuridine (EU). EdU and EU are respectively used as alternatives for (3)H-thymidine in UDS and for (3)H-uridine in RRS. Based on these alkyne-nucleosides and an integrated image analyser, we have developed a semi-automated assay system for NER-activity. We demonstrate the utility of this system for NER-activity assessments of lymphoblastoid samples as well as primary fibroblasts. Potential use of the system for large-scale siRNA-screening for novel NER defects as well as for routine XP diagnosis are also considered.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"DNA repair, 9(5), pp.506-516; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"20171149","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.dnarep.2010.01.015","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2010 Elsevier B.V. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11032547","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"15687864","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"15687856","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakazawa, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Shunichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lehmann, Alan R"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogi, Tomoo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"DNA_Ogi.pdf","filesize":[{"value":"534.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DNA_Ogi.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15191/files/DNA_Ogi.pdf"},"version_id":"66ddbbe2-e848-4f07-a3b7-5e6c25c302ce"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cockayne syndrome (CS)","subitem_subject_scheme":"Other"},{"subitem_subject":"Nucleotide excision repair (NER)","subitem_subject_scheme":"Other"},{"subitem_subject":"Recovery of RNA synthesis (RRS)","subitem_subject_scheme":"Other"},{"subitem_subject":"Transcription-coupled repair (TCR)","subitem_subject_scheme":"Other"},{"subitem_subject":"Unscheduled DNA synthesis (UDS)","subitem_subject_scheme":"Other"},{"subitem_subject":"Xeroderma pigmentosum (XP)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives."}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-15"},"publish_date":"2010-03-15","publish_status":"0","recid":"15191","relation_version_is_last":true,"title":["A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:51:34.135953+00:00"}