{"created":"2023-05-15T16:40:48.215519+00:00","id":15208,"links":{},"metadata":{"_buckets":{"deposit":"2319c492-27a8-4877-8f8f-defd302dc936"},"_deposit":{"created_by":2,"id":"15208","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15208"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015208","sets":["8:9"]},"author_link":["55940","55937","55943","55941","55938","55942","55946","55945","55939","55944"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"1146","bibliographicPageStart":"1139","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"Cellular and molecular neurobiology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hemorrhagic transformation is a major complication associated with tissue plasminogen activator (tPA) therapy for ischemic stroke. We studied the effect of tPA on the blood-brain barrier (BBB) function with our in vitro monolayer model generated using rat brain microvascular endothelial cells subjected either to normoxia or to hypoxia/reoxygenation (H/R) with or without the administration of tPA. The barrier function was evaluated by the transendothelial electrical resistance (TEER), the permeability of sodium fluorescein and Evans' blue-albumin (EBA), and the uptake of lucifer yellow (LY). The permeability of sodium fluorescein and EBA was used as an index of paracellular and transcellular transport, respectively. The administration of tPA increased the permeability of EBA and the uptake of LY under normoxia. It enhanced the increase in the permeability of both sodium fluorescein and EBA, the decrease in the TEER, and the disruption in the expression of ZO-1 under H/R conditions. Administration of tPA could cause an increase in the transcellular transport under normoxia, and both the transcellular and paracellular transport of the BBB under H/R conditions in vitro. Even in humans, tPA may lead to an opening of the BBB under non-ischemic conditions and have an additional effect on the ischemia-induced BBB disruption.","subitem_description_type":"Abstract"}]},"item_2_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The original publication is available at www.springerlink.com","subitem_description_type":"Other"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Cellular and molecular neurobiology, 28(8), pp.1139-1146; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Netherlands"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18629628","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10571-008-9294-x","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Springer Science+Business Media, LLC 2008"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10622352","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02724340","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"15736830","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hiu, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Sinsuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Kentaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitagawa, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsutsumi, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakubo, Junichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honda, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suyama, Kazuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagata, Izumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niwa, Masami"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"CMN28_1139.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CMN28_1139.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15208/files/CMN28_1139.pdf"},"version_id":"d961b63a-42b2-4489-a8ab-be7957fdf2a7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Blood-brain barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"Brain capillary endothelial cells (rat)","subitem_subject_scheme":"Other"},{"subitem_subject":"Hypoxia/reoxygenation (in vitro)","subitem_subject_scheme":"Other"},{"subitem_subject":"Tight junction","subitem_subject_scheme":"Other"},{"subitem_subject":"Tissue plasminogen activator","subitem_subject_scheme":"Other"},{"subitem_subject":"Transendothelial electrical resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"Transendothelial permeability","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Tissue plasminogen activator enhances the hypoxia/reoxygenation-induced impairment of the blood-brain barrier in a primary culture of rat brain endothelial cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tissue plasminogen activator enhances the hypoxia/reoxygenation-induced impairment of the blood-brain barrier in a primary culture of rat brain endothelial cells."}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-24"},"publish_date":"2010-03-24","publish_status":"0","recid":"15208","relation_version_is_last":true,"title":["Tissue plasminogen activator enhances the hypoxia/reoxygenation-induced impairment of the blood-brain barrier in a primary culture of rat brain endothelial cells."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:57:32.229718+00:00"}