{"created":"2023-05-15T16:40:49.395649+00:00","id":15236,"links":{},"metadata":{"_buckets":{"deposit":"24d4932d-1aa0-418e-b682-f8bcb3f94377"},"_deposit":{"created_by":2,"id":"15236","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15236"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015236","sets":["27:28"]},"author_link":["56121","56120","56125","56124","56123","56118","56126","56119","56122"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"544","bibliographicPageStart":"538","bibliographicVolumeNumber":"53","bibliographic_titles":[{"bibliographic_title":"Archives of oral biology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Porphyromonas gingivalis, a major etiological bacterium of periodontal diseases, produces a unique lysine-specific cysteine proteinase (Lys-gingipain, Kgp) implicated in the virulence of this organism. Our observations show the expression of a catalytically active recombinant Kgp in a P. gingivalis Kgp-null mutant and the restoration of its functions by the use of a shuttle plasmid vector stable in P. gingivalis. The Kgp-expressing mutant exhibited a similar catalytic activity to that of the wild-type strain. This mutant also restored the ability to form black-pigmented colonies on blood agar plates and to generate a 19-kDa haemoglobin receptor protein responsible for haemoglobin binding. In order to establish the importance of the active-site Cys residue and elucidate its role in bacterial black pigmentation we constructed three Kgp mutants with changed potential active-site Cys residues. The cells expressing a single mutation (C476A) showed the high Kgp activity and the black pigmentation. In contrast, the cells expressing the single mutant (C477A) and the double mutant (C476A/C477A) exhibited neither Kgp activity nor black pigmentation. These results indicate that the 477th Cys residue is essential for both the Kgp activity and the black pigmentation of P. gingivalis.","subitem_description_type":"Abstract"}]},"item_2_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博（医歯薬）甲第139号 学位授与年月日:平成20年2月27日","subitem_description_type":"Other"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Archives of oral biology, 53(6), pp.538-544; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ltd"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18295742","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.archoralbio.2008.01.004","subitem_relation_type_select":"DOI"}}]},"item_2_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://hdl.handle.net/10069/16965"}]}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2008 Elsevier Ltd All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00548457","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00039969","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishida, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hu, Jinping"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai, Eiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kadowaki, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukuba, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Yuzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Koji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Kuniaki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"AOB53_538.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AOB53_538.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15236/files/AOB53_538.pdf"},"version_id":"1af96e5c-1fb1-47f4-8870-5698708fced7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Arg-gingipain","subitem_subject_scheme":"Other"},{"subitem_subject":"Lys-gingipain","subitem_subject_scheme":"Other"},{"subitem_subject":"Periodontal diseases","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_scheme":"Other"},{"subitem_subject":"Recombinant expression","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system."}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-04-15"},"publish_date":"2010-04-15","publish_status":"0","recid":"15236","relation_version_is_last":true,"title":["Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:06:56.421273+00:00"}