{"created":"2023-05-15T16:40:49.734287+00:00","id":15244,"links":{},"metadata":{"_buckets":{"deposit":"ea57cc92-1592-45f8-8e94-53991b6addb2"},"_deposit":{"created_by":2,"id":"15244","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15244"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015244","sets":["8:9"]},"author_link":["56182","56189","56184","56188","56186","56187","56178","56183","56179","56181","56180","56185"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"217","bibliographicPageStart":"210","bibliographicVolumeNumber":"82","bibliographic_titles":[{"bibliographic_title":"Life sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pirfenidone (5-methyl-1-phenyl-2-(1H)-pyridone) is a novel anti-fibrotic and anti-inflammatory agent that inhibits the progression of fibrosis in animal models and patients with idiopathic pulmonary fibrosis (IPF). Heat shock protein (HSP) 47, a collagen-specific molecular chaperone, is involved in the processing and/or secretion of procollagen and plays an important role in the pathogenesis of IPF. The present study evaluated the in vitro effects of pirfenidone on expression of HSP47 and collagen type I in cultured normal human lung fibroblasts (NHLF). Expression levels of HSP47 and collagen type I in NHLF stimulated by transforming growth factor (TGF)-beta1 were evaluated genetically, immunologically and immunocytochemically. Treatment with TGF-beta1 stimulated both mRNA and protein expressions of both HSP47 and collagen type I in NHLF, and pirfenidone significantly inhibited this TGF-beta1-enhanced expression in a dose-dependent manner. We concluded that the anti-fibrotic effect of pirfenidone may be mediated not only through direct inhibition of collagen type I expression but also at least partly through inhibition of HSP47 expression in lung fibroblasts, with a resultant reduction of collagen synthesis in lung fibrosis.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Life sciences, 82(3-4), pp.210-217; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18093617","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.lfs.2007.11.003","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2007 Elsevier Inc. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00717011","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00243205","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakayama, Seiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukae, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakamoto, Noriho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kakugawa, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshioka, Sumako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Soda, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oku, Hisashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urata, Yoshie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Takahito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kubota, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagata, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kohno, Shigeru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"LifSci82_210.pdf","filesize":[{"value":"664.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"LifSci82_210.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15244/files/LifSci82_210.pdf"},"version_id":"7307c37e-2765-481c-9c2a-7eac9de9826c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Collagen type I","subitem_subject_scheme":"Other"},{"subitem_subject":"Fibroblast","subitem_subject_scheme":"Other"},{"subitem_subject":"Heat shock protein 47 (HSP47)","subitem_subject_scheme":"Other"},{"subitem_subject":"Idiopathic pulmonary fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Lung fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Pirfenidone","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Pirfenidone inhibits the expression of HSP47 in TGF-beta1-stimulated human lung fibroblasts.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Pirfenidone inhibits the expression of HSP47 in TGF-beta1-stimulated human lung fibroblasts."}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-04-20"},"publish_date":"2010-04-20","publish_status":"0","recid":"15244","relation_version_is_last":true,"title":["Pirfenidone inhibits the expression of HSP47 in TGF-beta1-stimulated human lung fibroblasts."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:00:48.453625+00:00"}