{"created":"2023-05-15T16:40:52.285480+00:00","id":15303,"links":{},"metadata":{"_buckets":{"deposit":"7b18c701-be13-48da-aa1e-30d807322b96"},"_deposit":{"created_by":2,"id":"15303","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15303"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015303","sets":["73:74"]},"author_link":["56570","56568","56564","56562","56563","56565","56569","56566","56567"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"78","bibliographicPageStart":"74","bibliographicVolumeNumber":"665","bibliographic_titles":[{"bibliographic_title":"Analytica chimica acta"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to evaluate the chemiluminescence (CL) reagents for selective detection of reactive oxygen species (ROS), we comprehensively measured the CL responses of 20 CL reagents (three luminol derivatives, two imidazopyrazinone derivatives, eight lophine derivatives, six acridinium ester derivatives and lucigenin) against six types of ROS (superoxide anion: O(2)(*-), hydroxyl radical: *OH, hydrogen peroxide: H(2)O(2), hypochlorite anion: ClO(-), singlet oxygen: (1)O(2), and nitric oxide: NO). As a result of the screening, it was found that nine CL reagents selectively detected O(2)(*-) while one CL reagent selectively detected *OH. However, no CL reagent had selectivity on the detection of H(2)O(2), ClO(-), (1)O(2) and NO. Our screening results could help to select the most suitable CL reagent for selective determination of different ROS. As an application study, 4-methoxyphenyl-10-methylacridinium-9-carboxylate (MMAC), one of the acridinium ester derivatives, showed high selectivity on the detection of O(2)(*-), and thus was applied to the assay of superoxide dismutase (SOD) activity. The dynamic range and detection limit of the developed CL assay were 0.1-10 and 0.06 U mL(-1), respectively. Significant correlation (r=0.997) was observed between the results by the CL assay using MMAC and the spectrophotometric assay using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Analytica chimica acta, 665(1), pp.74-78; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"20381693","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.aca.2010.03.025","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Crown copyright © 2010 Published by Elsevier B.V."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00524834","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00032670","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18734324","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamaguchi, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohba, Yoshihito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kohno, Maiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuda, Toshinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takadate, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Kenichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"ACA665_74.pdf","filesize":[{"value":"162.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ACA665_74.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15303/files/ACA665_74.pdf"},"version_id":"90f46cd9-aff0-4f30-ad20-a2d068ac7dcd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Acridinium ester","subitem_subject_scheme":"Other"},{"subitem_subject":"Chemiluminescent probe","subitem_subject_scheme":"Other"},{"subitem_subject":"Reactive oxygen species","subitem_subject_scheme":"Other"},{"subitem_subject":"Screening","subitem_subject_scheme":"Other"},{"subitem_subject":"Superoxide dismutase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species."}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-01"},"publish_date":"2010-06-01","publish_status":"0","recid":"15303","relation_version_is_last":true,"title":["Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:07:21.410317+00:00"}