{"created":"2023-05-15T16:40:52.892067+00:00","id":15317,"links":{},"metadata":{"_buckets":{"deposit":"5b384c41-8862-46c1-b673-9706f8bb9573"},"_deposit":{"created_by":2,"id":"15317","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15317"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015317","sets":["25:26"]},"author_link":["56640","56642","56639","56641","56638","56644","56643"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-05-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"494","bibliographicPageStart":"489","bibliographicVolumeNumber":"396","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is known that dehydroepiandrosterone (DHEA) inhibits a phenotypic switch in vascular smooth muscle cells (VSMC) induced by platelet-derived growth factor (PDGF)-BB. However, the mechanism behind the effect of DHEA on VSMC is not clear. Previously we reported that low molecular weight-protein tyrosine phosphatase (LMW-PTP) dephosphorylates PDGF receptor (PDGFR)-beta via a redox-dependent mechanism involving glutathione (GSH)/glutaredoxin (GRX)1. Here we demonstrate that the redox regulation of PDGFR-beta is involved in the effect of DHEA on VSMC. DHEA suppressed the PDGF-BB-dependent phosphorylation of PDGFR-beta. As expected, DHEA increased the levels of GSH and GRX1, and the GSH/GRX1 system maintained the redox state of LMW-PTP. Down-regulation of the expression of LMW-PTP using siRNA restored the suppression of PDGFR-beta-phosphorylation by DHEA. A promoter analysis of GRX1 and gamma-glutamylcysteine synthetase (gamma-GCS), a rate-limiting enzyme of GSH synthesis, showed that DHEA up-regulated the transcriptional activity at the peroxisome proliferator-activated receptor (PPAR) response element, suggesting PPARalpha plays a role in the induction of GRX1 and gamma-GCS expression by DHEA. In conclusion, the redox regulation of PDGFR-beta is involved in the suppressive effect of DHEA on VSMC proliferation through the up-regulation of GSH/GRX system.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biochemical and biophysical research communications, 396(2), pp.489-494; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"20420805","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2010.04.125","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2010 Elsevier Inc. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"10902104","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Urata, Yoshishige"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakatsu, Miho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yodoi, Junji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eto, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akishita, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Takahito"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"BBRC396_489.pdf","filesize":[{"value":"527.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BBRC396_489.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15317/files/BBRC396_489.pdf"},"version_id":"60b5e9fd-8645-46ac-b642-e81fe6eab0b1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Dehydroepiandrosterone","subitem_subject_scheme":"Other"},{"subitem_subject":"Glutaredoxin 1","subitem_subject_scheme":"Other"},{"subitem_subject":"Glutathione","subitem_subject_scheme":"Other"},{"subitem_subject":"Low molecular weight-protein tyrosine phosphatase","subitem_subject_scheme":"Other"},{"subitem_subject":"Platelet-derived growth factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Vascular smooth muscle cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation."}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-09"},"publish_date":"2010-06-09","publish_status":"0","recid":"15317","relation_version_is_last":true,"title":["DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:06:36.029192+00:00"}