{"created":"2023-05-15T16:40:56.580056+00:00","id":15403,"links":{},"metadata":{"_buckets":{"deposit":"77eb8350-f0ce-4f2e-a567-9f6a646fca50"},"_deposit":{"created_by":2,"id":"15403","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15403"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015403","sets":["175:315"]},"author_link":["57041","57045","57044","57040","57043","57042"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"117","bibliographicPageStart":"113","bibliographicVolumeNumber":"110","bibliographic_titles":[{"bibliographic_title":"Journal of bioscience and bioengineering"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The effects of fucose-containing sulfated polysaccharides, ascophyllan and fucoidan, isolated from the brown alga Ascophyllum nodosum, on the growth of various cell lines (MDCK, Vero, PtK(1), CHO, HeLa, and XC) were investigated. In a colony formation assay, ascophyllan and fucoidan showed potent cytotoxic effects on Vero and XC cells, while other cell lines were relatively resistant to these polysaccharides. Almost no significant effects of these polysaccharides were observed in the cell lines tested using the Alamar blue cytotoxicity assay over 48 h with varying initial cell densities (2500-20,000 cells/well) in growth medium. Interestingly, a significant growth promoting effect of ascophyllan on MDCK cells was observed, whereas treatment with fucoidan showed growth suppressive effects on this cell line under the same experimental conditions. These results suggest that ascophyllan is distinguishable from fucoidan in terms of their bioactivities. This is the first report of the growth promoting effects of a sulfated fucan on a mammalian cell line under normal growth conditions.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of bioscience and bioengineering, 110(1), pp.113-117; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"20541128","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jbiosc.2010.01.007","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2010 The Society for Biotechnology, Japan Published by Elsevier B.V."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11307678","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13891723","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13474421","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jiang, Zedong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okimura, Takasi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokose, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamasaki, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Kenichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oda, Tatsuya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"JBB110_113.pdf","filesize":[{"value":"991.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JBB110_113.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15403/files/JBB110_113.pdf"},"version_id":"7b046b2f-399f-48ef-bff8-3913ba4574b5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ascophyllan","subitem_subject_scheme":"Other"},{"subitem_subject":"Ascophyllum nodosum","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytotoxicity","subitem_subject_scheme":"Other"},{"subitem_subject":"Fucoidan","subitem_subject_scheme":"Other"},{"subitem_subject":"Growth promotion","subitem_subject_scheme":"Other"},{"subitem_subject":"MDCK cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Sulfated fucan","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of sulfated fucan, ascophyllan, from the brown Alga Ascophyllum nodosum on various cell lines: a comparative study on ascophyllan and fucoidan.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of sulfated fucan, ascophyllan, from the brown Alga Ascophyllum nodosum on various cell lines: a comparative study on ascophyllan and fucoidan."}]},"item_type_id":"2","owner":"2","path":["315"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-07"},"publish_date":"2010-07-07","publish_status":"0","recid":"15403","relation_version_is_last":true,"title":["Effects of sulfated fucan, ascophyllan, from the brown Alga Ascophyllum nodosum on various cell lines: a comparative study on ascophyllan and fucoidan."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:49:43.654508+00:00"}