{"created":"2023-05-15T16:30:25.658068+00:00","id":1549,"links":{},"metadata":{"_buckets":{"deposit":"68a3f842-d46f-4e7c-b422-6c057a5983d3"},"_deposit":{"created_by":2,"id":"1549","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1549"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001549","sets":["10:11"]},"author_link":["7448","7449","7447","7446"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"20","bibliographicPageStart":"11","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"Microbiology and Immunology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The type IX secretion system (T9SS) was originally discovered in Porphyromonas gingivalis, one of the pathogenic bacteria associated with periodontal disease and is now known to be present in many members\n of the phylum Bacteroidetes. The T9SS secretes a number of potent virulence factors,including the highly hydrolytic proteases called gingipains, across the outer membrane in P.gingivalis. To understand the entire machinery of T9SS, an exhaustive search for T9SS-related genes in P. gingivalis using the mariner family transposon (Tn) and Tn-seq analysis was performed. Seven hundred and two Tn insertion sites in Tn mutants with no colony pigmentation that is associated with Lys-gingipain (Kgp) defectiveness were determined, and it was found that the Tn was inserted in the kgp gene and 54 T9SS-related candidate genes. Thirty-three out of the 54 genes were already known as T9SS-related genes. Furthermore, deletion mutant analysis of the remaining 21 genes revealed that they were not related to the T9SS. The 33 T9SS-related genes include a gene for PGN_0297, \nwhich was found to be associated with the T9SS components PorK and PorN. A PGN_0297 gene deletion mutant was constructed, and it was found that the mutant showed no colony pigmentation, hemagglutination or gingipain activities, indicating that PGN_0297 was an essential component of the T9SS. The 33 genes did not include the six genes (gppX, omp17, porY, rfa, sigP and wzx) that were also reported as T9SS-related genes. gppX deletion and insertion mutants were constructed, and it was found that they did not show deficiency in the T9SS.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Microbiology and Immunology, 63(1), pp.11-20; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons Australia, Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/1348-0421.12665","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium,provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855600","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Naito, Mariko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tominaga, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shoji, Mikio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Koji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"MicImm63_11.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MicImm63_11.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1549/files/MicImm63_11.pdf"},"version_id":"5e8c3829-1a7e-4b14-8db9-02ecd2dbb30c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"colony pigmentation","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_scheme":"Other"},{"subitem_subject":"Tn-seq analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"type IX secretion system","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"PGN_0297 is an essential component of the type IX secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PGN_0297 is an essential component of the type IX secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-15"},"publish_date":"2019-02-15","publish_status":"0","recid":"1549","relation_version_is_last":true,"title":["PGN_0297 is an essential component of the type IX secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:00:27.883836+00:00"}