{"created":"2023-05-15T16:41:17.969094+00:00","id":15894,"links":{},"metadata":{"_buckets":{"deposit":"84a7a290-82c9-4ca8-92bd-66de070b32ce"},"_deposit":{"created_by":2,"id":"15894","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15894"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015894","sets":["10:11"]},"author_link":["59397","59401","59400","59399","59402","59398","59403"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"4054","bibliographicPageStart":"4047","bibliographicVolumeNumber":"151","bibliographic_titles":[{"bibliographic_title":"Endocrinology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Graves-like hyperthyroidism is induced in BALB/c mice by immunization with adenovirus expressing the human TSH receptor (TSHR) A-subunit (amino acids 1-289). However, because of nonidentity between the human and mouse TSHR ( approximately 87% amino acid homology), we compared the responses of mice immunized with adenoviruses expressing either the mouse or the human TSHR A-subunit. Wild-type (wt) BALB/c mice immunized with the mouse A-subunit developed neither TSHR antibodies (measured by flow cytometry) nor thyroid lymphocytic infiltration. However, wt C57BL/6 mice developed sparse intrathyroidal lymphocyte infiltration without antibody production. Depletion of naturally occurring regulatory CD4(+)CD25(+) T cells had little effect. These results indicate the inability to break tolerance to the mouse TSHR in wt mice. In contrast, TSHR knockout (KO) BALB/c mice generated mouse TSHR antibodies in response to mouse A-subunit immunization and augmented human TSHR antibody response to human A-subunit immunization. Thyroid-stimulating antibody titers measured in a functional bioassay were comparable in human A-subunit immunized wt mice and in TSHR KO mice immunized with either the mouse or human A-subunit. In conclusion, immune response to the mouse TSHR is readily induced in TSHR KO but not in wt mice. Only in the former does immunization with adenovirus expressing the mouse A-subunit generate antibodies capable of activating the mouse TSHR. TSHR KO mice are, therefore, of value for future studies dissecting the autoimmune response to the mouse TSHR.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Endocrinology, 151(8), pp.4047-4054; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Endocrine Society"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"20555026","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1210/en.2010-0315","subitem_relation_type_select":"DOI"}}]},"item_2_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://hdl.handle.net/10069/26720"}]}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2010 by The Endocrine Society"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00635074","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00137227","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19457170","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakahara, Mami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitsutake, Norisato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakamoto, Hikaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chen, Chun-Rong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rapoport, Basil"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"McLachlan, Sandra M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagayama, Yuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"Endo151_4047.pdf","filesize":[{"value":"937.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Endo151_4047.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15894/files/Endo151_4047.pdf"},"version_id":"743f3321-b9b7-4816-bc72-be9209c76571"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Graves’ disease","subitem_subject_scheme":"Other"},{"subitem_subject":"thyrotropin receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"knockout mice","subitem_subject_scheme":"Other"},{"subitem_subject":"thyroid autoimmunity","subitem_subject_scheme":"Other"},{"subitem_subject":"cross-reactivity","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Enhanced response to mouse thyroid-stimulating hormone (TSH) receptor immunization in TSH receptor-knockout mice.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Enhanced response to mouse thyroid-stimulating hormone (TSH) receptor immunization in TSH receptor-knockout mice."}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-09-01"},"publish_date":"2011-09-01","publish_status":"0","recid":"15894","relation_version_is_last":true,"title":["Enhanced response to mouse thyroid-stimulating hormone (TSH) receptor immunization in TSH receptor-knockout mice."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:29:57.908023+00:00"}