{"created":"2023-05-15T16:41:19.135179+00:00","id":15922,"links":{},"metadata":{"_buckets":{"deposit":"48c39739-5ad3-4736-b500-e312462e9cc6"},"_deposit":{"created_by":2,"id":"15922","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"15922"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00015922","sets":["10:11"]},"author_link":["59539","59541","59540","59542","59538"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"e129","bibliographicPageStart":"e129","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Nucleic acids research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An ataxia-telangiectasia mutated (ATM)-dependent DNA damage signal is amplified through the interaction of various factors, which are recruited to the chromatin regions with DNA double-strand breaks. Spatial and temporal regulation of such factors is analysed by fluorescence microscopy in combination with laser micro-irradiation. Here we describe a novel and simple technique for micro-irradiation that does not require a laser source. Cells were labelled with BrdU for 48-72 h, covered with porous polycarbonate membranes, and exposed to UVC. All BrdU-labelled cells showed localized foci of phosphorylated ATM, phosphorylated histone H2AX, MDC1 and 53BP1 upon irradiation, showing that these foci were induced irrespective of the cell-cycle phase. They were also detectable in nucleotide excision repair-defective XPA cells labelled with BrdU, indicating that the foci did not reflect an excision repair-related process. Furthermore, an ATM-specific inhibitor significantly attenuated the foci formation, and disappearance of the foci was significantly abrogated in non-homologous end-joining-defective cells. Thus, it can be concluded that micro-irradiation generated DNA double-strand breaks in BrdU-sensitized cells. The present technique should accelerate research in the fields of DNA damage response, DNA repair and DNA recombination, as it provides more chances to perform micro-irradiation experiments without any specific equipment.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nucleic acids research, 38(12), pp.e129; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"20385602","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/nar/gkq226","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2010. Published by Oxford University Press."},{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1203820X","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13624962","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Keiji"}],"nameIdentifiers":[{"nameIdentifier":"59538","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamauchi, Motohiro"}],"nameIdentifiers":[{"nameIdentifier":"59539","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oka, Yasuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"59540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masatoshi"}],"nameIdentifiers":[{"nameIdentifier":"59541","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamashita, Shunichi"}],"nameIdentifiers":[{"nameIdentifier":"59542","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"NAR38_129.pdf","filesize":[{"value":"4.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"NAR38_129.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/15922/files/NAR38_129.pdf"},"version_id":"737adc48-b45e-4634-bbb5-003d04032298"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks."}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-09-10"},"publish_date":"2010-09-10","publish_status":"0","recid":"15922","relation_version_is_last":true,"title":["A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T21:05:32.423928+00:00"}