{"created":"2023-05-15T16:29:25.327653+00:00","id":161,"links":{},"metadata":{"_buckets":{"deposit":"7ecf83a0-998b-458f-b7f5-e5e331f66b77"},"_deposit":{"created_by":2,"id":"161","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"161"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000161","sets":["10:11"]},"author_link":["788","787","784","786","789","785"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-11-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"19149","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Rab44 is a large Rab GTPase that contains an amino-terminal EF-hand domain, a coiled-coil domain, and a carboxyl-terminal Rab GTPase domain.However, the roles of the EF-hand and coiled-coil domains remain unclear. Here, we constructed various deletion and point mutants of human Rab44. When overexpressed in HeLa cells, the wild-type Rab44 (hWT) formed ring-like structures, and partially localised to lysosomes. The dominant negative mutant, hT847N, localised to lysosomes and the cytosol, while the constitutively active mutant, hQ892L, \nformed ring-like structures, and partially localised to the plasma membrane and nuclei. The hΔEF, hΔcoil, and h826-1021 mutants also \nformed ring-like structures; however, their localisation patterns differed from hWT. Analysis of live imaging with LysoTracker revealed that the size of LysoTracker-positive vesicles was altered by all other mutations than the hC1019A and hΔEF. Treatment with ionomycin, a Ca2+ ionophore, induced the translocation of hWT and hΔcoil into the plasma membrane and cytosol, but had no effect on the localisation of the hΔEF and h826-1021 mutants. Thus, the EF- hand domain is likely required for the partial translocation of Rab44 to the plasma \nmembrane and cytosol following transient Ca2+ influx, and the coiled-coil domain appears to be important for localisation and organelle formation.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Scientific Reports, 10(1), art.no.19149; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-020-75897-7","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020, The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ogawa, Kohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kadowaki, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tokuhisa, Mitsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Umeda, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukuba, Takayuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-16"}],"displaytype":"detail","filename":"SciRep10_19149.pdf","filesize":[{"value":"6.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SciRep10_19149.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/161/files/SciRep10_19149.pdf"},"version_id":"66802468-8519-4e5b-9118-698670afad5e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Role of the EF-hand and coiled-coil domains of human Rab44 in localisation and organelle formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of the EF-hand and coiled-coil domains of human Rab44 in localisation and organelle formation"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-19"},"publish_date":"2020-11-19","publish_status":"0","recid":"161","relation_version_is_last":true,"title":["Role of the EF-hand and coiled-coil domains of human Rab44 in localisation and organelle formation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:21:02.720904+00:00"}