@article{oai:nagasaki-u.repo.nii.ac.jp:00016215,
 author = {Ishimoto, Hiroshi and Yanagihara, Katsunori and Araki, Nobuko and Mukae, Hiroshi and Sakamoto, Noriho and Izumikawa, Koichi and Seki, Masafumi and Miyazaki, Yoshitsugu and Hirakata, Yoichi and Mizuta, Yohei and Yasuda, Kenji and Kohno, Shigeru},
 issue = {4},
 journal = {Japanese Journal of Infectious Diseases},
 month = {Jul},
 note = {Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection., Japanese Journal of Infectious Diseases, 61(4), pp. 294-297; 2008},
 pages = {294--297},
 title = {Single-cell observation of phagocytosis by human blood dendritic cells},
 volume = {61},
 year = {2008}
}